Variable Colonization after Reciprocal Fecal Microbiota Transfer between Mice with Low and High Richness Microbiota.
Variable Colonization after Reciprocal Fecal Microbiota Transfer between Mice with Low and High Richness Microbiota.
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DOI：
10.3389/fmicb.2017.00196
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发表时间：
2017
影响因子：
5.2
通讯作者：
Franklin CL
中科院分区：
文献类型：
--
作者：
Ericsson AC;Personett AR;Turner G;Dorfmeyer RA;Franklin CL
肠道常驻微生物群的特征与人类健康和疾病易感性之间存在多种关联。动物模型提供了前瞻性测试这些相关性并评估因果关系的方法。实验性粪便微生物群转移（FMT），或将肠道微生物有意移植到用抗生素耗尽其原生微生物的受体小鼠中，是测试这些关系的常用方法。文献中很少记录通过此类程序进行微生物转移的真正完整性，特别是在含有不同丰富度和多样性的微生物种群的受体小鼠和供体小鼠之间相互转移微生物的情况下。此外，尚不清楚使用冷冻粪便内容物或盲肠内容物是否会带来转移结果的任何差异。在此，将具有不同丰富度和组成的不同肠道微生物群定植的小鼠组用于相互 FMT 研究，不同组接受由新鲜盲肠内容物、新鲜粪便或冷冻粪便制备的材料的转移。在整个研究过程中，每隔一段时间就使用靶向 16S rRNA 基因扩增子测序来表征微生物群。值得注意的是，尽管在移植前受体小鼠体内的微生物群已被相当程度地耗尽，但只有当相对丰富的供体材料被转移到最初殖民有较简单微生物群的小鼠时，供体特异性分类群才能可靠地定植于受体。目前尚不清楚这些差异是由于内源性受体微生物群的差异还是由于微生物因素在生命早期引起的宿主因素的差异。这些发现对于使用 FMT 前瞻性评估小鼠模型中肠道微生物群的影响的研究人员以及研究宿主-微生物相互作用及其对肠道屏障功能影响的研究人员具有实际意义。
Several associations have been made between characteristics of the resident gut microbiota and human health and disease susceptibility. Animal models provide the means to test these correlations prospectively and evaluate causality. Experimental fecal microbiota transfer (FMT), or the intentional transplantation of gut microbes into recipient mice depleted of their autochthonous microbes with antibiotics, is a commonly used method of testing these relationships. The true completeness of microbial transfer through such procedures is poorly documented in the literature, particularly in the context of reciprocal transfer of microbes between recipient and donor mice harboring microbial populations of differing richness and diversity. Moreover, it is unclear whether the use of frozen fecal contents or cecal contents would confer any difference in the outcomes of transfer. Herein, groups of mice colonized with distinct gut microbiota of differing richness and composition were used in a reciprocal FMT study, with different groups receiving transfer of material prepared from fresh cecal contents, fresh feces, or frozen feces. Targeted 16S rRNA gene amplicon sequencing was used at intervals throughout the study to characterize the microbiota. Notably, despite comparable depletion of the microbiota in recipient mice prior to transfer, donor-specific taxa reliably colonized recipients only when relatively rich donor material was transferred to mice originally colonized with a simpler microbiota. It is unclear whether these differences were due to differences in the endogenous recipient microbiota or host factors induced in early life by microbial factors. These findings are of practical import for researchers using FMT to prospectively assess the influence of the gut microbiota in mouse models, and to those studying host-microbial interactions and their influence on gut barrier function.