Improved derivatization technique for gas chromatography-mass spectrometry determination of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone in drinking water.
Improved derivatization technique for gas chromatography-mass spectrometry determination of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone in drinking water.
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改进的衍生化技术用于气相色谱-质谱法测定饮用水中的 3-氯-4-(二氯甲基)-5-羟基-2(5H)-呋喃酮。
DOI：
10.1016/j.aca.2009.07.035
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发表时间：
2009
影响因子：
6.2
通讯作者：
B. R. Gauthier
中科院分区：
文献类型：
--
作者：
C. Kubwabo;B. Stewart;Suzanne A. Gauthier;B. R. Gauthier
由于饮用水中 MX 浓度低、对 pH 变化高度敏感以及干扰衍生化和分析的基质效应，饮用水中 3-氯-4-(二氯甲基)-5-羟基-2(5H)-呋喃酮（Mutagen X 或 MX）的定量很困难。通常，MX 的定量涉及甲基化衍生化。我们提出了一种使用 N-甲基双三氟乙酰胺 (MBTFA) 进行 MX 的一步衍生化程序，并通过离子阱 GC/MS/MS 进行分析。新方法显着缩短了分析时间并提高了检测限。三氟酰化 MX（三氟乙酸-4-氯-3-二氯甲基-5-氧代-2-氢-呋喃-2-基酯）质谱中丰富且具有选择性的离子可以清晰地识别和定量该化合物，方法检测限为 7.7ngL−1，定量限为 24.4ngL−1。三氟酰化 MX 在过量的衍生剂中可稳定 30 天。新方法用于多种饮用水样品中MX的测定，浓度范围从未检出到517ngL−1；这些值与之前研究中获得的值相当。这种新的 MX 简化分析方法的开发是消毒副产物 (DBP) 研究领域向前迈出的重要一步，特别是考虑到最近科学界认识到卤化呋喃酮是新兴的饮用水污染物。分析能力的提高很可能是监测这些消毒副产物的决定性因素。
The quantification of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (Mutagen X or MX) in drinking water is difficult due to the low concentration of MX in drinking water, its high sensitivity to pH change, and matrix effects that interfere with the derivatization and analysis. Typically, the quantification of MX involves derivatization by methylation. We present a one-step derivatization procedure for MX using N-methyl-bis-trifluoroacetamide (MBTFA) and analysis by ion trap GC/MS/MS. The new method resulted in a significant reduction in analysis time, and improved detection limits. The abundant and selective ions in the mass spectrum of the trifluoroacylated MX (trifluoroacetic acid-4-chloro-3-dichloromethyl-5-oxo-2-hydro-furan-2-yl ester) allowed for a clear identification and quantification of the compound, with a method detection limit of 7.7ngL−1, and a limit of quantitation of 24.4ngL−1. The trifluoroacylated MX was shown to be stable for 30 days in an excess of the derivatization reagent. The new method was applied for the determination of MX in several drinking water samples, with a concentration range from not-detected to 517ngL−1; these values are comparable to those obtained in previous studies. The development of this new simplified analytical method for MX is an important step forward in the field of disinfection by-product (DBP) research, particularly in light of the recent scientific recognition of halogenated furanones as emerging drinking water contaminants. Increased analytical ability may well be a decisive factor in the monitoring of these disinfection by-products.
马萨诸塞州饮用水中的 3-Chloro-4-(二氯甲基)-5-羟基-2(5H)-呋喃酮 (MX) 和诱变活性。
影响因子：
10.4
作者：
Wright,JMichael;Schwartz,Joel;Vartiainen,Terttu;Mäki-Paakkanen,Jorma;Altshul,Larisa;Harrington,JosephJ;Dockery,DouglasW
通讯作者：
Dockery,DouglasW