AtRabF2b (Ara7) acts on the vacuolar trafficking pathway in tobacco leaf epidermal cells
AtRabF2b (Ara7) acts on the vacuolar trafficking pathway in tobacco leaf epidermal cells
复制标题
DOI：
10.1242/jcs.01564
复制
发表时间：
2004-12-15
影响因子：
4
通讯作者：
Hawes, C
中科院分区：
文献类型：
--
作者：
Kotzer, AM;Brandizzi, F;Hawes, C
Rab gtpase是细胞内分泌运输事件的普遍关键调节因子。特别是，rab5同源物与内吞事件和空泡途径有关。在这项研究中，我们利用活细胞成像方法研究了该家族成员AtRabF2b （Ara7）在烟草（Nicotiana tabacum）叶表皮细胞中的位置和功能。荧光标记AtRabF2b[wt]定位于泡前腔室和高尔基体，通过与这些腔室的荧光标记物共表达研究确定。破坏AtRabF2b功能的突变也会改变GTPase的亚细胞位置。此外，该蛋白与液泡靶向淀粉样蛋白绿色荧光蛋白（GFP）的共表达研究以及野生型AtRabF2b的拯救实验表明，AtRabF2b的显性阴性突变体使液泡标记物分泌到外质体中。我们的研究结果表明AtRabF2b在液泡运输途径中有明确的作用。
Rab GTPases are universal key regulators of intracellular secretory trafficking events. In particular, Rab 5 homologues have been implicated in endocytic events and in the vacuolar pathway. In this study, we investigate the location and function of a member of this family, AtRabF2b (Ara7) in tobacco (Nicotiana tabacum) leaf epidermal cells using a live cell imaging approach. Fluorescent-tagged AtRabF2b[wt] localized to the prevacuolar compartment and Golgi apparatus, as determined by coexpression studies with fluorescent markers for these compartments. Mutations that impair AtRabF2b function also alter the subcellular location of the GTPase. In addition, coexpression studies of the protein with the vacuole-targeted aleurain-green fluorescent protein (GFP) and rescue experiments with wild-type AtRabF2b indicate that the dominant-negative mutant of AtRabF2b causes the vacuolar marker to be secreted to the apoplast. Our results indicate a clear role of AtRabF2b in the vacuolar trafficking pathway.