Dissection and cytological mapping of barley chromosome 2H in the genetic background of common wheat.
Dissection and cytological mapping of barley chromosome 2H in the genetic background of common wheat.
复制标题
普通小麦遗传背景下大麦 2H 染色体的解剖和细胞学定位。
DOI：
10.1266/ggs.86.231
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发表时间：
2011
影响因子：
1.1
通讯作者：
T. Endo
中科院分区：
文献类型：
--
作者：
G. Joshi;S. Nasuda;T. Endo
我们利用杀配子染色体2C和3C（SAT）对大麦2 H和普通小麦进行了解剖。Gc染色体诱导染色体断裂，导致携带单体Gc染色体的普通小麦2 H附加系的后代中的染色体畸变。我们进行了原位杂交，以选择植物进行结构重排异常2 H染色体，其特征在于连续C-显带和原位杂交。建立了66个2 H染色体畸变的普通小麦离体细胞系。异常2 H染色体为缺失、易位或复杂的结构改变。它们的断裂点分布在短臂（2 HS）、着丝粒（2 HC）和长臂（2 HL）上，2 HS/2 HC/2 HL的比例大致为2：1：2。我们使用115个2 H染色体特异性EST标记对66个解剖系进行了PCR分析。根据PCR结果，我们构建了2 H染色体的物理或细胞学图谱，该图谱以异常2 H染色体的断裂点为界分为34个区域。在2 HS和2 HL中分别有47个和68个标记物。我们比较了2 H细胞学地图与以前报道的2 H遗传图谱，使用44个标记，用于共同构建两个地图。两个标测图中远端区域的标记顺序相同，但近端区域的标记顺序在两个标测图之间有些矛盾。我们发现，在遗传图谱中分布相当均匀的标记，实际上集中在两个臂的远端区域所揭示的细胞学图谱。我们还认识到EST标记或基因丰富的区域在2 HL间质区略端粒。
We used gametocidal (Gc) chromosomes 2C and 3C(SAT) to dissect barley 2H added to common wheat. The Gc chromosome induces chromosomal breakage resulting in chromosomal aberrations in the progeny of the 2H addition line of common wheat carrying the monosomic Gc chromosome. We conducted in situ hybridization to select plants carrying structurally rearranged aberrant 2H chromosomes and characterized them by sequential C-banding and in situ hybridization. We established 66 dissection lines of common wheat carrying single aberrant 2H chromosomes. The aberrant 2H chromosomes were of either deletion or translocation or complicated structural change. Their breakpoints were distributed in the short arm (2HS), centromere (2HC) and the long arm (2HL) at a rough 2HS/2HC/2HL ratio of 2:1:2. We conducted PCR analysis of the 66 dissection lines using 115 EST markers specific to chromosome 2H. Based on the PCR result, we constructed a physical or cytological map of chromosome 2H that were divided into 34 regions separated by the breakpoints of the aberrant 2H chromosomes. Forty-seven markers were present in 2HS and 68 in 2HL. We compared the 2H cytological map with a previously reported 2H genetic map using 44 markers that were used in common to construct both maps. The order of markers in the distal region was the same on both maps but that in the proximal region was somewhat contradictory between the two maps. We found that the markers distributed rather evenly in the genetic map were actually concentrated in the distal regions of both arms as revealed by the cytological map. We also recognized an EST-marker or gene-rich region in the 2HL interstitial region slightly to the telomere.
影响因子：
3.8
作者：
Sato, K.;Nankaku, N.;Takeda, K.
通讯作者：
Takeda, K.