Cerebrospinal Fluid and Serum Nitric Oxide Levels in Asphyxiated Newborns
Cerebrospinal Fluid and Serum Nitric Oxide Levels in Asphyxiated Newborns
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DOI：
10.1159/000014159
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发表时间：
1999-09
期刊：
影响因子：
2.5
通讯作者：
E. Ergenekon;K. Gücüyener;D. Erbaş;Fatih Süheyl Ezgü;Y. Atalay
中科院分区：
文献类型：
--
作者：
E. Ergenekon;K. Gücüyener;D. Erbaş;Fatih Süheyl Ezgü;Y. Atalay
缺氧缺血性脑病（HIE）是由细胞因子和一氧化氮（NO）释放引起的一系列事件的结果，随后发生自由基损伤。为了探讨一氧化氮在窒息新生儿中的作用，我们检测了17例HIE新生儿血清和脑脊液中一氧化氮的含量。妊娠37周及以上的婴儿因Sarnat和Sarnat被分为轻度、中度和重度HIE。在24至72小时之间获得的样品立即在-70°C冷冻，直到用Sievers NOA测量时。轻度5例，中度6例，重度6例，重度4例合并多系统累及。中度和重度HIE组脑脊液NO水平明显高于轻度HIE组（p = 0.028和p = 0.018）。我们的研究结果表明，在窒息后24 ~ 72 h，脑脊液中NO水平随HIE严重程度的增加而升高。根据动物实验，这是诱导型NO合成酶被激活的时期，可能导致神经毒性，这可能通过干预来预防。
Hypoxic-ischemic encephalopathy (HIE) is the result of a chain of events caused mainly by cytokines and nitric oxide (NO) release, which is later on followed by free oxygen radical injury. To investigate NO involvement in asphyxiated newborns, serum and cerebrospinal fluid (CSF) values of NO levels in 17 neonates with HIE were detected. Infants at or above 37 weeks of gestation were classified to have mild, moderate and severe HIE due to Sarnat and Sarnat. Samples obtained between 24 and 72 h of life were immediately frozen at –70°C till the time of measurement by Sievers NOA. Five patients had mild, 6 patients had moderate and 6 patients had severe HIE, 4 in the severe HIE group also had multisystem involvement. The CSF NO levels were significantly higher in moderate and severe HIE groups compared to the mild HIE group (p = 0.028 and p = 0.018 respectively). Our results show that NO level increases in CSF with the severity of HIE between 24 and 72 h following asphyxia. According to the animal work, this is the time period where inducible NO synthase gets activated and could cause neurotoxicity, which might perhaps be prevented by interventions.