Characterisation of Pasteurella multocida isolates from pigs with pneumonia in Korea
Characterisation of Pasteurella multocida isolates from pigs with pneumonia in Korea
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DOI：
10.1186/s12917-019-1861-5
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发表时间：
2019-04-25
影响因子：
2.6
通讯作者：
Kim, Ha-Young
中科院分区：
文献类型：
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作者：
Kim, Jongho;Kim, Jong Wan;Kim, Ha-Young
背景多杀性巴氏杆菌（Pasteurella multocida）是世界范围内造成猪重大经济损失的病原菌。在临床患病猪中，大多数多杀性巴氏杆菌分离株被表征为多杀性亚种、生物变型2或3以及荚膜类型A或D;然而，关于韩国多杀性巴氏杆菌分离株的亚种、生物变型和其他荚膜类型的信息很少。在这里，我们使用表型和基因型特征以及与最低抑菌浓度相关的数据，提供了韩国肺炎猪中有关多杀性巴氏杆菌的长期信息。ResultsThe overall prevalence of P. multocida between 2008 and 2016 is 16.8%（240/1430），85%（204/240）的多杀性巴氏杆菌分离株与其他呼吸道病原体共感染。在240株分离株中，166株被纳入本研究;所有这些多杀性巴氏杆菌分离株均被表征为多杀性亚种，最常见的表型为生物变型3（68.7%; n=114）和荚膜A型（69.9%; n=116）。此外，还鉴定了三种F型荚膜分离株，这是韩国首次报告此类分离株。所有生物变型1和2分离株分别为F型和A型荚膜。毒力相关基因的分布是可变的，所有荚膜A型和D型分离株分别携带pmHAS和hsf-1（P
BackgroundPasteurella multocida is responsible for significant economic losses in pigs worldwide. In clinically diseased pigs, most P. multocida isolates are characterised as subspecies multocida, biovar 2 or 3 and capsular type A or D; however, there is little information regarding subspecies, biovars, and other capsular types of P. multocida isolates in Korea. Here, we provided information covering an extended time period regarding P. multocida in pigs with pneumonia in Korea using phenotypic and genotypic characterisations and data associated with the minimum inhibitory concentrations.ResultsThe overall prevalence of P. multocida between 2008 and 2016 was 16.8% (240/1430), with 85% of the P. multocida isolates (204/240) coinfected with other respiratory pathogens. Of the 240 isolates, 166 were included in this study; all of these P. multocida isolates were characterised as subspecies multocida and the most prevalent phenotypes were represented by biovar 3 (68.7%; n=114) and capsular type A (69.9%; n=116). Additionally, three capsular type F isolates were identified, with this representing the first report of such isolates in Korea. All biovar 1 and 2 isolates were capsular types F and A, respectively. The virulence-associated gene distribution was variable; all capsular type A and D isolates harboured pmHAS and hsf-1, respectively (P