Characterization of a clonal Sertoli cell line using adult PyLT transgenic mice
Characterization of a clonal Sertoli cell line using adult PyLT transgenic mice
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DOI：
10.1095/biolreprod58.2.591
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发表时间：
1998-02-01
影响因子：
3.6
通讯作者：
Pointis, G
中科院分区：
文献类型：
--
作者：
Bourdon, V;Lablack, A;Pointis, G
本文报道了从性成熟多瘤病毒大T（PyLT）转基因小鼠中分离并鉴定了一株克隆性Sertoli细胞系（42 GPA 9）。42 GPA 9细胞系表达与正常支持细胞相关的生化特征。通过逆转录-聚合酶链反应和Western印迹分析检测转铁蛋白、硫酸化糖蛋白-2和c-kif配体。酶谱分析表明，42 GPA 9细胞系分泌组织型纤溶酶原激活剂，这些细胞还保留FSH受体，这表明它们对促性腺激素具有特异性反应（形态学和吞噬细胞变化，刺激cAMP产生）和FSH受体mRNA的检测，42 GPA 9细胞系的另一个原始方面是其在融合时形成紧密连接的能力，如通过电子显微镜研究和紧密连接的免疫定位所证明的。连接相关蛋白质闭锁小带1. 42 GPA 9细胞系保留了正常支持细胞的几个重要特征，可能有助于进一步研究支持细胞的行为和支持细胞-生殖细胞在成熟睾丸中的相互作用。
In the present study we report the isolation and characterization of a clonal Sertoli cell line (42GPA9) from sexually mature polyoma virus large T (PyLT) transgenic mice, The cells multiplied indefinitely and expressed large T antigen. The 42GPA9 cell line expressed biochemical features associated with normal Sertoli cells. Transferrin, sulfated glycoprotein-2, and the ligand of c-kif were detected by reverse transcription-polymerase chain reactions and Western blot analyses. Zymographic analysis indicated that the 42GPA9 cell line secreted tissue-type plasminogen activator, These cells also retained FSH receptors as suggested by their specific responsiveness to the gonadotropin (morphological and phagocytic changes, stimulation of cAMP production) and the detection of FSH receptor mRNAs, Another original aspect of the 42GPA9 cell line is its ability to form tight junctions at confluency as demonstrated by electron microscopic study and immunolocalization of the tight junction-associated protein zonula occludens 1. The 42GPA9 cell line, which has retained several important hallmarks of normal Sertoli cells, may prove useful for further studies on Sertoli cell behavior and on Sertoli-germ cell interactions in the mature testis.