Tissue engineering applications to vascular bypass graft development: The use of adipose-derived stem cells
Tissue engineering applications to vascular bypass graft development: The use of adipose-derived stem cells
复制标题
DOI：
10.1016/j.jvs.2007.02.046
复制
发表时间：
2007-06-01
影响因子：
4.3
通讯作者：
Tulenko, Thomas
中科院分区：
文献类型：
--
作者：
DiMuzio, Paul;Tulenko, Thomas
蓬勃发展的血管组织工程领域为创造实用和成功的小直径动脉旁路移植术带来了希望。自体细胞和支架的许多创造性组合存在，以及同样长的微环境线索列表，用于创建功能性动脉导管。这篇综述概述了我们使用腹壁脂肪作为血管组织工程自体干细胞来源的工作，特别关注这种干细胞的可用性和分化成内皮样细胞的能力。在49例接受择期外周血管手术的患者中，从抽脂获得的脂肪中获得了大量的成体干细胞。尽管来自糖尿病患者的脂肪产生的干细胞明显减少，但分离的效果似乎不受高龄、肥胖、肾衰竭或血管疾病的影响。此外，这些脂肪来源的干细胞在体外暴露于生长因子（内皮细胞生长补充剂和血管内皮生长因子）和生理性剪切应力时获得了几种形态和分子内皮表型。综上所述，这些研究表明脂肪似乎是用于血管组织工程的自体干细胞的可行来源。
The burgeoning field of vascular tissue engineering holds promise for the creation of a practical and successful small-diameter arterial bypass graft. Many creative combinations of autologous cells and scaffolds exist along with an equally long list of microenvironmental cues used to create a functional arterial conduit. This review outlines our work using abdominal wall fat as a source of autologous stem cells for vascular tissue engineering, focusing specifically on this stem cell's availability and potency to differentiate into endothelial-like cells. In a series of 49 patients undergoing elective peripheral vascular surgery, an abundant quantity of adult stem cells was harvested from fat obtained by liposuction. The efficacy of the isolation did not appear influenced by advanced age, obesity, renal failure, or vascular disease, although fat from diabetic patients yielded significantly less stem cells. In addition, these adipose-derived stem cells acquired several morphologic and molecular endothelial phenotypes when exposed to growth factors (endothelial cell growth supplement and vascular endothelial growth factor) and physiologic shear stress in vitro. Taken together, these studies suggest that fat appears to be a viable source of autologous stem cells for use in vascular tissue engineering.