MORPHOLOGY OF STORED, REJUVENATED HUMAN ERYTHROCYTES
MORPHOLOGY OF STORED, REJUVENATED HUMAN ERYTHROCYTES
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DOI：
10.1111/j.1423-0410.1975.tb02756.x
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发表时间：
1975-01-01
期刊：
影响因子：
2.7
通讯作者：
MANALO, FW
中科院分区：
文献类型：
--
作者：
USRY, RT;MOORE, GL;MANALO, FW
将全血在4°C下储存42天。从第21天开始，每隔7天，用磷酸盐、肌苷、葡萄糖丙酮酸盐和腺嘌呤溶液对等分试样进行复活。在返老还童之前和之后均采集样品用于ATP测定和红细胞形态学（通过SEM）。返老还童提高了所有ATP水平超过100%的起始值，并显着增加了百分比的细胞在盘状的形状所测量的形态指数，表明返老还童不仅提高了细胞的代谢水平，但导致部分恢复到新鲜的细胞形态。
Whole blood was stored at 4°C for 42 days. At 7‐day intervals, starting with day 21, aliquots were rejuvenated with a phosphate, inosine, glucose pyruvate, and adenine solution. Samples were taken for ATP assays and red cell morphology (by SEM) both before and after rejuvenation. Rejuvenation elevated all ATP levels in excess of 100% of the starting values and significantly increased the percent of cells in the discoid shape as measured by their morphological index, showing that rejuvenation not only raises the cell's metabolic level but causes partial reversion to fresh cell morphology.