SURFACE DENSITY DETERMINATION IN MEMBRANES BY FLUORESCENCE ENERGY-TRANSFER
SURFACE DENSITY DETERMINATION IN MEMBRANES BY FLUORESCENCE ENERGY-TRANSFER
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DOI：
10.1021/bi00617a025
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发表时间：
1978-01-01
期刊：
影响因子：
2.9
通讯作者：
STRYER, L
中科院分区：
文献类型：
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作者：
FUNG, BKK;STRYER, L
研究了合成膜囊泡中荧光标记磷脂之间的能量转移，以确定转移效率对随机阵列中能量受体表面密度的依赖性。本研究中使用的荧光磷脂是1，5-丹酰磷脂酰乙醇胺（1，5-DPE）、2，6-丹酰磷脂酰乙醇胺（2，6-DPE）、2，5-丹酰磷脂酰乙醇胺（2，5-DPE）和曙红磷脂酰乙醇胺（EPE）。将荧光探针掺入由磷脂酰胆碱形成的单双层囊泡中。导致50%转移的能量受体的表面密度对于从1，5-DPE转移到EPE、从2，5-DPE转移到EPE、从2，6-DPE转移到EPE和从2，6-DPE转移到2，5-DPE分别为0.0066、0.0057、0.01和0.024个受体/磷脂。观察到的
Energy transfer between fluorescent-labeled phospholipids in synthetic membrane vesicles was investigated to ascertain the dependence of the transfer efficiency on the surface density of the energy acceptor in a random array. The fluorescent phospholipids used in this study were 1, 5-dansyl-phosphatidylethanolamine (1, 5-DPE), 2, 6-dansylphosphatidylethanolamine (2, 6-DPE), 2, 5-dansylphosphatidylethanolamine (2, 5-DPE), and eosinphosphatidylethanolamine (EPE). The fluorescent probes were incorporated into single bilayer vesicles formed from phosphatidylcholine. The surface densities of energy acceptor resulting in 50% transfer were 0.0066, 0.0057, 0.01, and 0.024 acceptors per phospholipid for transfer from 1, 5-DPE to EPE, 2, 5-DPE to EPE, 2, 6-DPE to EPE, and 2, 6-DPE to 2, 5-DPE, respectively. The observed