CHARACTERIZATION OF THE MAJOR EPSTEIN-BARR VIRUS-SPECIFIC RNA IN BURKITT LYMPHOMA-DERIVED CELLS
CHARACTERIZATION OF THE MAJOR EPSTEIN-BARR VIRUS-SPECIFIC RNA IN BURKITT LYMPHOMA-DERIVED CELLS
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DOI：
10.1128/jvi.41.2.376-389.1982
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发表时间：
1982-01-01
影响因子：
5.4
通讯作者：
RYMO, L
中科院分区：
文献类型：
--
作者：
ARRAND, JR;RYMO, L
从5个人淋巴样细胞系和伯基特淋巴瘤活检组织中制备的细胞质RNA进行了体外放射性标记，并与克隆的B95-8爱泼斯坦-巴尔病毒DNA EcoRI限制性内切酶片段杂交。这些细胞中最丰富的细胞质RNA种类是由EcoRI J片段定义的基因组的一个小区域指定的。详细的定位实验精确地将这些转录本定位在EcoRI J片段最右侧1/3的序列中。DNA测序表明，爱泼斯坦-巴尔病毒基因组的这一区域无法编码蛋白质。主要的早期转录本包括2种非聚腺苷化的RNA，每种。apprx。长度为170个核苷酸。它们都是从同一条DNA链转录而来，并显示出显著的序列同源性。这2个小RNA的编码序列含有RNA聚合酶III的潜在基因内控制区。
Cytoplasmic RNA prepared from 5 human lymphoid cell lines and a Burkitt lymphoma biopsy was radioactively labeled in vitro and hybridized to cloned EcoRI restriction endonuclease fragments of B95-8 Epstein-Barr virus DNA. The most abundant cytoplasmic RNA species in such cells is specified by a small region of the genome defined by the EcoRI J fragment. Detailed mapping experiments precisely localized these transcripts within the sequence of the rightmost 1/3 of the EcoRI J fragment. DNA sequencing suggested that this region of the Epstein-Barr virus genome is unable to code for protein. The major early transcripts consisted of 2 non-polyadenylated RNA species, each .apprx. 170 nucleotides in length. They were both transcribed off the same strand of the DNA and showed significant sequence homology with each other. The coding sequences of the 2 small RNA contained potential intragenic control regions for RNA polymerase III.