Viscoelastic adlayers of collagen and lysozyme studied using quartz crystal microbalance with dissipation monitoring
Viscoelastic adlayers of collagen and lysozyme studied using quartz crystal microbalance with dissipation monitoring
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使用石英晶体微天平和耗散监测研究胶原蛋白和溶菌酶的粘弹性吸附层
DOI：
10.1016/j.ijbiomac.2010.02.005
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发表时间：
2010
影响因子：
8.2
通讯作者：
Araki Y
中科院分区：
文献类型：
--
作者：
Nezu T;Taira M;Saitoh S;Sasaki K;Araki Y
本研究用QCM-D方法研究了蛋白质分子结构对其吸附行为和粘弹性的影响。吸附测量结果表明，球形溶菌酶迅速吸附在金表面，形成一个坚硬的单层，传感器的振荡频率急剧下降（Δf），吸附层的能量耗散有很小的增加（ΔD）。然而，纤维小牛皮胶原蛋白（CSC）在无盐条件下，在pH为3的条件下，通过两步缓慢地吸附在同一表面上，形成厚而软的多层（大Δf和ΔD）。对于刚性聚集的CSC，没有观察到两步吸附。该研究清楚地表明，聚合物结构不仅强烈影响吸附的发展，而且影响粘弹性层的性能。
In this study the effects of molecular structure of proteins on their adsorption behaviour and viscoelasticity are investigated using a QCM-D method. The adsorption measurements show that spherical lysozyme is rapidly adsorbed on the gold surface as a stiff monolayer, as indicated by a sharp drop in the oscillation frequency (Δf) of the sensor, and by a very small increase in the energy dissipation of the adlayer (ΔD). Fibrous calfskin collagen (CSC) is, however, adsorbed on the same surface rather slowly in two steps to form a thick and soft multilayer (large Δf and ΔD) at pH 3 in salt-free conditions. The two-step adsorption is not observed for stiffly aggregated CSC. This study clearly demonstrates that the polymer structure strongly affects not only how adsorption develops but also the viscoelastic properties of the adlayer.