Anti-inflammatory activity of polysaccharide purified from AMG-assistant extract of Ecklonia cava in LPS-stimulated RAW 264.7 macrophages
Anti-inflammatory activity of polysaccharide purified from AMG-assistant extract of Ecklonia cava in LPS-stimulated RAW 264.7 macrophages
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DOI：
10.1016/j.carbpol.2011.01.052
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发表时间：
2011-04-22
影响因子：
11.2
通讯作者：
Jeon, You-Jin
中科院分区：
文献类型：
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作者：
Kang, Sung-Myung;Kim, Kil-Nam;Jeon, You-Jin
海藻岩藻聚糖硫酸酯是一种具有多种生物活性的海洋硫酸多糖。我们评估了来自Ecklonia cava的硫酸化多糖对LPS刺激的RAW 264.7细胞中的抗炎活性的潜在活性。在本研究中，E. Cava被五种糖酶和五种蛋白酶水解。我们的研究结果表明，AMG提取物具有最高的产量，并发挥最深刻的抑制作用，对NO的生产。为了鉴定活性成分，我们进行了微滤膜，乙醇添加分离，阴离子交换和凝胶渗透色谱法;纯化的多糖（PPS），随后获得。PPS显著抑制LPS刺激的RAW 264.7细胞NO、PGE（2）的产生，并抑制诱导型一氧化氮合酶（iNOS）和环氧合酶（考克斯2）的表达。因此，PPS通过抑制iNOS和考克斯-2来抑制NO和PGE（2）的产生。这些结果表明PPS的抗炎活性可能归因于抗炎剂的调节。皇冠版权所有（C）2011由爱思唯尔有限公司出版。保留所有权利。
Algal fucoidan is a marine sulfated polysaccharide that evidences a variety of biological activities. We assessed the potential activity of the sulfated polysaccharide from Ecklonia cava, on anti-inflammatory activity in LPS-stimulated RAW 264.7 cells. In this study, E. cava was hydrolyzed by five carbohydrases and five proteases. Our findings demonstrated that the AMG extract has the highest yield and exerts the most profound inhibitory effects against NO production. To identify the active compounds, we conducted micro-filtration membrane, ethanol-added separation, and anion exchange and gel permeation chromatography; the purified polysaccharide (PPS) was subsequently obtained. PPS significantly inhibited NO production, prostaglandin-E-2 (PGE(2)) production and suppressed inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW 264.7 cells. Thus, PPS inhibited NO and PGE(2) production via the inhibition of iNOS and COX-2. These results indicate that the anti-inflammatory activity of PPS may be attributable to the modulation of anti-inflammatory agents. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.