Collagen synthesis promoting pullulan-PEI-ascorbic acid conjugate as an efficient anti-cancer gene delivery vector
Collagen synthesis promoting pullulan-PEI-ascorbic acid conjugate as an efficient anti-cancer gene delivery vector
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DOI：
10.1016/j.carbpol.2015.03.023
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发表时间：
2015-08-01
影响因子：
11.2
通讯作者：
Rekha, M. R.
中科院分区：
文献类型：
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作者：
Ambattu, Lizebona August;Rekha, M. R.
合成了阳离子化普鲁兰多糖（pullulan-PEI; PP），并进一步用抗氧化剂抗坏血酸（PPAA）以不同比例进行改性。以4：1的最佳比例形成的纳米复合物的尺寸在150 nm以内，并且具有9-14 mV的zeta电位。该比例的纳米复合物用于进一步研究。在血清存在下测定这些纳米复合物的细胞内化和转染效率。发现内化和转染效率不受胎牛血清存在的影响。另一个有趣的观察结果是发现该聚合物具有胶原合成促进性质。定量这些聚合物的胶原合成效果，并观察到PPAA 3促进最高。通过评估C6大鼠神经胶质瘤细胞中的p53基因表达来评价转染效率，并且通过流式细胞术将细胞死亡定量为96%，从而确立了该聚合物的高功效。(C)2015爱思唯尔有限公司版权所有。
Cationized pullulan (pullulan-PEI; PP) was synthesized and further modified with an anti-oxidant molecule, ascorbic acid (PPAA) at various ratios. The nanoplexes formed at an optimum ratio of 4:1 was within a size of 150 nm and had a zeta potential of 9-14 mV. The nanoplexes at this ratio was used for further investigations. The cell internalization and transfection efficiency of these nanoplexes were determined in presence of serum. The internalization and transfection efficiency were found to be unaffected by the presence of fetal bovine serum. Another interesting observation was that this polymer was found to have collagen synthesis promoting property. The collagen synthesis effect of these polymers was quantified and observed that PPAA3 promoted the highest. Transfection efficiency was evaluated by assessing the p53 gene expression in C6 rat glioma cells and cell death was quantified to be 96% by flow cytometry, thus establishing the high efficacy of this polymer. (C) 2015 Elsevier Ltd. All rights reserved.