Tannic Acid-Lung Fluid Assemblies Promote Interaction and Delivery of Drugs to Lung Cancer Cells.
Tannic Acid-Lung Fluid Assemblies Promote Interaction and Delivery of Drugs to Lung Cancer Cells.
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DOI：
10.3390/pharmaceutics10030111
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发表时间：
2018-08-01
期刊：
影响因子：
5.4
通讯作者：
Yallapu MM
中科院分区：
文献类型：
--
作者：
Hatami E;Nagesh PKB;Chowdhury P;Chauhan SC;Jaggi M;Samarasinghe AE;Yallapu MM
肺癌（LC）是美国男性和女性死亡的主要原因之一。单宁酸（TA）是一种水溶性多酚，具有广泛的生物活性。TA作为生物材料和药物载体领域的一种有前途的化合物受到了广泛的关注。肺液（LF）是药物分布到肺部的主要屏障。因此，本研究的目的是检查TA与LF的相互作用，以通过肺部递送有效递送抗癌药物分子。通过荧光光谱中的荧光猝灭来揭示TA对LF蛋白的吸附程度。在TA-LF复合物中LF的存在通过在1653 cm−1处的蛋白质峰的存在而被注意到。蛋白质斑点和SDS-PAGE分析均证实在所用的所有TA浓度下LF蛋白质复合。通过透射电子显微镜（TEM）分析观察到稳定的颗粒TA-LF复合物形成。通过动态光散射（DLS）测定的络合图案表明，它取决于溶液的pH值。LF在TA-LF复合物中的存在程度表明其在LC细胞系中的相互作用行为。这种上级相互作用提供了包封在TA-LF复合物纳米制剂中的药物的增强的抗癌活性。我们的研究结果表明，TA结合LF和形成自组装，这深刻地增强与LC细胞的相互作用。这项研究表明，TA是一种新型的药物载体，如吉西他滨，卡铂和伊立替康。
Lung cancer (LC) is one of the leading causes of death in both men and women in the United States. Tannic acid (TA), a water-soluble polyphenol, exhibits a wide range of biological activities. TA has received much attention as a promising compound in the biomaterial and drug delivery fields. Lung fluid (LF) is a major barrier for distribution of drugs to the lungs. Therefore, the purpose of this study was to examine TA interaction with LF for effective delivery of anti-cancer drug molecules via pulmonary delivery. The extent of adsorption of LF proteins by TA was revealed by fluorescence quenching in fluorescence spectroscopy. The presence of LF in TA-LF complexes was noticed by the presence of protein peaks at 1653 cm−1. Both protein dot and SDS-PAGE analysis confirmed LF protein complexation at all TA concentrations employed. A stable particle TA-LF complex formation was observed through transmission electron microscopy (TEM) analysis. The complexation pattern measured by dynamic light scattering (DLS) indicated that it varies depending on the pH of the solutions. The degree of LF presence in TA-LF complexes signifies its interactive behavior in LC cell lines. Such superior interaction offered an enhanced anti-cancer activity of drugs encapsulated in TA-LF complex nanoformulations. Our results indicate that TA binds to LF and forms self-assemblies, which profoundly enhance interaction with LC cells. This study demonstrated that TA is a novel carrier for pharmaceutical drugs such as gemcitabine, carboplatin, and irinotecan.