Isolation of natural cultures of anaerobic fungi and indigenously associated methanogens from herbivores and their bioconversion of lignocellulosic materials to methane
Isolation of natural cultures of anaerobic fungi and indigenously associated methanogens from herbivores and their bioconversion of lignocellulosic materials to methane
复制标题
从食草动物中分离厌氧真菌和本地相关产甲烷菌的自然培养物及其将木质纤维素材料生物转化为甲烷
DOI：
10.1016/j.biortech.2011.06.026
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发表时间：
2011-09-01
影响因子：
11.4
通讯作者：
Zhu, Wei-Yun
中科院分区：
文献类型：
--
作者：
Jin, Wei;Cheng, Yan-Fen;Zhu, Wei-Yun
本研究旨在从草食动物中获得天然厌氧真菌及其伴生产甲烷菌，并研究其降解木质纤维素为甲烷的能力。用Hungate滚管技术获得8个自然培养物。经显微镜鉴定，真菌分别属于梨囊菌属（Piromyces）、厌氧酵母属（Anaeromyces）和新美鞭菌属（Neocallimastix），产甲烷菌属（Methanobrebactorspp.）16 S rRNA基因测序用稻草进行的离体研究表明，这些培养物降解33.5-48.3%的底物，产生0.33-0.84 mmol/（100 ml培养物）甲烷。进一步选择两种培养物降解不同木质纤维素材料的能力，并可产生0.38-1.27 mmol/（100 ml培养物）甲烷。当甲烷菌被抑制时，培养物的木质纤维素降解能力显著降低。总之，本研究获得了具有高纤维降解能力的产甲烷菌的厌氧真菌天然培养物，这些培养物在木质纤维素降解和甲烷生产中具有工业应用潜力。(C)2011爱思唯尔有限公司保留所有权利。
This study aimed to obtain natural cultures of anaerobic fungi and their indigenously associated rnethanogens from herbivores and investigate their ability to degrade lignocelluloses to methane. Eight natural cultures were obtained by Hungate roll tube technique. The fungi were identified as belonging to Piromyces, Anaeromyces and Neocallimastix respectively by microscopy, and the methanogens as Methanobrevibactor spp. by 16S rRNA gene sequencing. In vitro studies with rice straw showed that these cultures degraded 33.5-48.3% substrate and produced 0.33-0.84 mmol/(100 ml culture) methane. Two cultures were further selected for their ability to degrade different lignocellulosic materials and could produce 0.38-1.27 mmol/(100 ml culture) methane. When methanogens were inhibited, the lignocellulose-degrading ability of cultures significantly reduced. In conclusion, natural cultures of anaerobic fungi with indigenously associated methanogens with high fiber degradation ability were obtained, and these cultures may have the potential in industrial use in lignocelluloses degradation and methane production. (C) 2011 Elsevier Ltd. All rights reserved.