Silencing of GbANS reduces cotton resistance to Verticillium dahliae through decreased ROS scavenging during the pathogen invasion process
Silencing of GbANS reduces cotton resistance to Verticillium dahliae through decreased ROS scavenging during the pathogen invasion process
复制标题
GbANS 沉默可通过减少病原体入侵过程中的 ROS 清除来降低棉花对大丽黄萎病的抗性
DOI：
10.1007/s11240-018-1457-y
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发表时间：
2018-11-01
影响因子：
3
通讯作者：
Song, Chun-Peng
中科院分区：
文献类型：
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作者：
Long, Lu;Zhao, Jing-Ruo;Song, Chun-Peng
花色苷是植物次生代谢产物，在植物适应逆境中起重要作用。花青苷生物合成途径在高等植物中是保守的。先前的研究揭示了花青素在天然彩色棉中的重要作用。然而，花色素苷在棉花与病原菌互作中的作用却知之甚少。本研究从海岛棉中分离到一个病原菌诱导的基因，该基因编码具有双加氧酶结构的花青素合成酶蛋白（GbANS）。GbANS在有色组织中优先表达。GbANS的沉默显著降低了花青素的产生，以及棉花对大丽轮枝菌的抗性。生化研究表明，GbANS沉默棉花积累更多的过氧化氢相比，控制植物在V.dahliae入侵过程中。这种过氧化氢的积累与入侵部位周围细胞死亡的增加相对应，这反过来又加速了大丽轮枝菌的感染。总而言之，我们发现GbANS有助于棉花花色苷的生物合成，而花色苷正调节棉花对大丽轮枝菌的抗性。
Anthocyanins are secondary metabolites that play important roles in plant adaption to adverse environments. The anthocyanin biosynthetic pathway is conserved in high plants. Previous studies revealed the significant role of anthocyanins in natural-colorized cotton. However, little is known about the involvement of anthocyanins in the interaction of cotton and pathogen. In this study, a pathogen-induced gene was isolated from Gossypium barbadense that encodes an anthocyanidin synthase protein (GbANS) with dioxygenase structures. GbANS was preferentially expressed in colored tissue. Silencing of GbANS significantly reduced the production of anthocyanins, as well as the cotton’s resistance to Verticillium dahliae. Biochemical studies revealed that GbANS-silenced cotton accumulated more hydrogen peroxide compared to control plants during the V. dahliae invasion process. This accumulation of hydrogen peroxide corresponded with increased cell death around the invasion sites, which in turn accelerated the V. dahliae infection. Taken together, we found that GbANS contributes to the biosynthesis of anthocyanins in cotton and anthocyanins positively regulate cotton’s resistance to V. dahliae.