The prognostic significance of eukaryotic elongation factor 1 alpha-2 in non-small cell lung cancer.
The prognostic significance of eukaryotic elongation factor 1 alpha-2 in non-small cell lung cancer.
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发表时间：
2014-02
影响因子：
2
通讯作者：
Masaki Kawamura;C. Endo;A. Sakurada;F. Hoshi;H. Notsuda;T. Kondo
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作者：
Masaki Kawamura;C. Endo;A. Sakurada;F. Hoshi;H. Notsuda;T. Kondo
背景真核细胞延伸因子1 α-2（eEF 1A 2）最近被证明是肺癌的假定癌基因。材料与方法我们分析了69例原发性非小细胞肺癌（NSCLC）中eEF 1A 2的表达及其预后意义。我们还使用RNA干扰抑制eEF 1A 2表达，然后分析5个腺癌细胞系的细胞增殖、迁移和侵袭。结果eEF 1A 2蛋白阳性表达率为84.1%。eEF 1A 2免疫组化阴性表达是一个独立的预后因素，并与淋巴结转移显著相关。eEF 1A 2蛋白表达与mRNA表达无明显相关性。在所检测的5个细胞系中，转染eEF 1A 2 siRNA仅在一个细胞系中抑制细胞迁移，而不改变细胞增殖和侵袭。结论eEF 1A 2免疫组化阴性提示NSCLC预后不良。这一结果的机制不能通过细胞增殖、迁移和侵袭试验来阐明。
BACKGROUND Eukaryotic elongation factor 1 alpha-2 (eEF1A2) has been recently shown to be a putative oncogene of lung cancer. MATERIALS AND METHODS We analyzed the expression and prognostic significance of eEF1A2 in 69 primary non-small cell lung cancer (NSCLC) cases. We also suppressed eEF1A2 expression using RNA interference and then analyzed cell proliferation, migration and invasion of five adenocarcinoma cell lines. RESULTS eEF1A2 protein expression was positive in 84.1%. Negative immunostaining for eEF1A2 was shown to be an independent prognostic factor and significantly correlated with lymph node metastasis. There was no significant correlation between eEF1A2 protein and mRNA expression levels. Among the five examined cell lines, transfection of eEF1A2 siRNA inhibited cell migration in only one cell line while it did not change cell proliferation and invasion. CONCLUSION Negative immunostaining of eEF1A2 predicted for poor prognosis of NSCLC. The mechanism of this result could not be elucidated by cell proliferation, migration and invasion assays.