Altered expression of circular RNAs in human placental chorionic plate-derived mesenchymal stem cells pretreated with hypoxia
Altered expression of circular RNAs in human placental chorionic plate-derived mesenchymal stem cells pretreated with hypoxia
复制标题
缺氧预处理的人胎盘绒毛膜板间充质干细胞中环状RNA表达的改变
DOI：
10.1002/jcla.22825
复制
发表时间：
2019-03-01
影响因子：
2.7
通讯作者：
Wu, Shaoqing
中科院分区：
文献类型：
--
作者：
Sun, Xunsha;Jin, Yulin;Wu, Shaoqing
背景低氧预适应改变了间充质干细胞（MSCs）的生物学特性。目前尚不清楚这一过程是否对MSC中的环状RNA（circRNA）有影响。方法从同一胎盘组织中分离培养人胎盘绒毛膜板间充质干细胞（hpcpMSCs），分为缺氧预处理组（缺氧组）和正常培养组（常氧组）。采用比较circRNA芯片分析两组circRNA的表达，并通过定量逆转录聚合酶链反应（qRT-PCR）进行验证。结果低氧组与常氧组比较，共发现102个差异表达的circRNA（倍数变化>1.5倍，P < 0.05）。通过qRT-PCR的circRNA表达水平与通过微阵列分析评估的那些一致。基因本体（GO）分析表明，这些差异表达的circRNA的靶基因的功能主要涉及细胞发育及其分化和调控。京都基因和基因组百科全书（KEGG）通路分析显示，在癌症中转录调控的错误和丝裂原活化蛋白激酶（MAPK）信号通路最为显著。MAPK信号通路被认为是缺氧触发的核心调控通路。结论低氧预适应可引起MSCs特异性circRNA表达的改变。这一发现为进一步探讨低氧预处理对骨髓间充质干细胞特征性改变的机制提供了依据。
Background Hypoxic preconditioning alters the biological properties of mesenchymal stem cells (MSCs). It is not known whether this process has an effect on circular RNAs (circRNAs) in MSCs. Methods Human placental chorionic plate-derived MSCs (hpcpMSCs) isolated from the same placentae were classed into two groups: hypoxic pretreated (hypoxia) group and normally cultured (normoxia) group. The comparative circRNA microarray analysis was used to determine circRNAs expression and verified by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) in the two groups. Results One hundred and two differentially expressed circRNAs in the hypoxia group were found compared to that in the normoxia group (fold change >1.5-fold and P < 0.05). The expression levels of circRNAs by qRT-PCR were consistent with those evaluated by microarray analysis. Gene ontology (GO) analysis showed that the putative function of their target genes for those differentially expressed circRNAs was primarily involved in cell development and its differentiation and regulation. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that transcriptional misregulation in cancer and mitogen-activated protein kinase (MAPK) signaling pathway were the most significant. MAPK signaling pathway was found to be the core regulatory pathway triggered by hypoxia. Conclusions The results indicate that the altered expression of specific circRNAs in MSCs is associated with hypoxic preconditioning. This finding provides further exploration of underlying mechanisms of the characteristic changes of MSCs with hypoxic preconditioning.