Application of fast chlorophyll a fluorescence kinetics to probe action target of 3-acetyl-5-isopropyltetramic acid
Application of fast chlorophyll a fluorescence kinetics to probe action target of 3-acetyl-5-isopropyltetramic acid
复制标题
应用快速叶绿素a荧光动力学探测3-乙酰基-5-异丙基四甲酸作用靶点
DOI：
10.1016/j.envexpbot.2011.08.005
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发表时间：
2011-11-01
影响因子：
5.7
通讯作者：
Qiang, Sheng
中科院分区：
文献类型：
--
作者：
Chen, Shiguo;Zhou, Fengyan;Qiang, Sheng
3-乙酰基-5-异丙基特特拉姆酸（3-AIPTA）是一种特特拉姆酸衍生物，是植物毒素细氮藻酸的类似物。研究表明，3-AIPTA是一种多靶点的根和茎生长以及光合作用抑制剂。基于快速叶绿素荧光动力学，3-AIPTA通过与Q（B）竞争Q（B）结合位点而阻断PSII受体侧Q（A）以外的电子传递，但不影响PSII天线的供体侧和光捕获功能。此外，较高的3-AIPTA浓度也抑制PSI末端受体的减少。JIP测试和与[C-14]阿特拉津的竞争性取代实验表明，3-AIPTA与类阿特拉津PSII抑制剂的结合环境不同，但它们具有相同的作用靶点Q（B）位点。推测特特拉姆酸家族是一种新型的光合电子传递链抑制剂，其中3-AIPTA和tenuazonic acid属于天然产物。(C)2010 Elsevier B. V.保留所有权利。
3-Acetyl-5-isopropyltetramic acid (3-AIPTA), an analogue of the phytotoxin tenuazonic acid, is a tetramic acid derivate. It is demonstrated here that 3-AIPTA is a multi-target inhibitor of root and shoot growth as well as photosynthesis. Based on fast chlorophyll fluorescence kinetics, 3-AIPTA blocks electron transport beyond Q(A) on the acceptor side of PSII by competing with Q(B) for the Q(B)-binding site, but does not affect the donor side and the light harvesting function of the PSII antenna. Additionally, higher 3-AIPTA concentration also inhibits the reduction of end acceptors of PSI. Evidences from JIP-test and competitive replacement with [C-14]atrazine showed that 3-AIPTA, like tenuazonic acid, does not share the same binding environment with the atrazine-like PSII inhibitors although they possess the common action target of the Q(B)-site. It is deduced that tetramic acid families of natural products, where 3-AIPTA and tenuazonic acid belong to, is a novel inhibitor type of the photosynthetic electron transport chain. (C) 2010 Elsevier B.V. All rights reserved.