Techno-economic analysis of a plant-based platform for manufacturing antimicrobial proteins for food safety
Techno-economic analysis of a plant-based platform for manufacturing antimicrobial proteins for food safety
复制标题
DOI：
10.1002/btpr.2896
复制
发表时间：
2019-09-11
影响因子：
2.9
通讯作者：
McDonald, Karen A.
中科院分区：
文献类型：
--
作者：
McNulty, Matthew J.;Gleba, Yuri;McDonald, Karen A.
全球食源性疾病的持续报道和抗生素耐药细菌的流行要求采取新的干预措施来确保我们的食品安全。使用噬菌体来源的溶素和细菌素类抗菌蛋白处理各种食品已被证明可以在食品供应链的多个干预点预防高风险病原体。食品工业采用抗菌蛋白作为食品安全干预措施的最大障碍是使用当前基于发酵的方法的高生产成本。最近，植物已被证明可以产生抗菌蛋白，其积累量高达 3 g/kg 鲜重，并且具有对抗主要食源性病原体的活性。为了研究这个新颖平台的潜在经济优势和可扩展性，我们评估了一种高效的转基因植物生产工艺。开发了详细的过程模拟模型，以帮助确定研究和开发重点的经济“热点”，包括对生产成本影响最大的过程操作参数、单元操作、消耗品和/或原材料。我们的分析表明，在三种情况下，商业规模植物中抗菌蛋白的单位生产成本为 3.00-6.88 美元/克，这可以支持与传统食品安全处理相比具有竞争力的销售价格。
Continuous reports of foodborne illnesses worldwide and the prevalence of antibiotic-resistant bacteria mandate novel interventions to assure the safety of our food. Treatment of a variety of foods with bacteriophage-derived lysins and bacteriocin-class antimicrobial proteins has been shown to protect against high-risk pathogens at multiple intervention points along the food supply chain. The most significant barrier to the adoption of antimicrobial proteins as a food safety intervention by the food industry is the high production cost using current fermentation-based approaches. Recently, plants have been shown to produce antimicrobial proteins with accumulation as high as 3 g/kg fresh weight and with demonstrated activity against major foodborne pathogens. To investigate potential economic advantages and scalability of this novel platform, we evaluated a highly efficient transgenic plant-based production process. A detailed process simulation model was developed to help identify economic "hot spots" for research and development focus including process operating parameters, unit operations, consumables, and/or raw materials that have the most significant impact on production costs. Our analyses indicate that the unit production cost of antimicrobial proteins in plants at commercial scale for three scenarios is $3.00-6.88/g, which can support a competitive selling price to traditional food safety treatments.