Leaf ion homeostasis and plasma membrane H(+)-ATPase activity in Vicia faba change after extra calcium and potassium supply under salinity.
Leaf ion homeostasis and plasma membrane H(+)-ATPase activity in Vicia faba change after extra calcium and potassium supply under salinity.
复制标题
盐度下额外补钙、补钾后蚕豆叶片离子稳态及质膜H()-ATP酶活性的变化
DOI：
10.1016/j.plaphy.2014.06.010
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Mühling K-H
中科院分区：
文献类型：
--
作者：
Morgan S;Maity P J;Geilfus;C-M Lindberg S;Mühling K-H
植物中的盐胁迫影响质外体离子活性和细胞质离子稳态。钙或钾对盐度下叶片中离子划分的改善作用尚不完全清楚。为了阐明钙或钾的供应如何改善盐度下的离子稳态和 ATP 酶活性，在水培法中添加 5 mM CaSO4 或 10 mM K2SO4（含或不含 100 mM NaCl）7 天和 21 天。通过基于显微镜的比率成像，用俄勒冈绿右旋糖酐染料检测完整的第二上部叶子中的质外体 pH 值。使用落射荧光显微镜分别检测负载有 Fura-2、SBFI、PBFI 和 BCECF 乙酰氧基甲酯的原生质体中的胞质 Ca2+、Na+、K+ 活性和 pH。此外，还研究了总 Ca2+、Na+、K+ 浓度和生长参数。 ATPase水解活性随着时间的延长而增加，但在长期盐处理后下降。 7 天后，经过钙处理的植物的活性大幅增加，但经过钾处理的植物的活性降低。在 7 d 和 21 d 期间，钙供应量增加 Vmax，并且 ATPase 活性随盐度以非竞争性方式增加。盐度 21 天后，钾供应反而与 Na+ 竞争性降低活性，对 Km 和 Vmax 产生不同的影响。已证实较高的 ATP 酶活性与质外体酸化、胞质碱化和低胞质 [Na+] 有关，因此，这可能是额外钙改善芽和叶生长的解释。
Salt stress in plants impacts apoplastic ion activities and cytosolic ionic homeostasis. The ameliorating effects exerted by calcium or potassium on compartmentation of ions in leaves under salinity are not fully understood. To clarify how calcium or potassium supply could ameliorate ion homeostasis and ATPase activities under salinity, 5 mM CaSO4or 10 mM K2SO4were added with, or without, 100 mM NaCl for 7 d and 21 d toViciafabagrown in hydroponics. The apoplastic pH was detected with Oregon Green dextran dye in intact second-uppermost leaves by microscopy-based ratio imaging. The cytosolic Ca2+, Na+, K+activities and pH were detected in protoplasts loaded with the acetoxy methyl-esters of Fura-2, SBFI, PBFI and BCECF, respectively, using epi-fluorescence microscopy. Furthermore, total Ca2+, Na+, K+concentrations and growth parameters were investigated. The ATPase hydrolyzing activity increased with time, but decreased after long salinity treatment. The activity largely increased in calcium-treated plants, but was depressed in potassium-treated plants after 7 d. The calcium supply increasedVmax, and the ATPase activity increased with salinity in a non-competitive way for 7 d and 21 d. The potassium supply instead decreased activity competitively with Na+, after 21 d of salinity, with different effects onKm andVmax. The confirmed higher ATPase activity was related with apoplast acidification, cytosol alkalinization and low cytosolic [Na+], and thus, might be an explanation why extra calcium improved shoot and leaf growth.
影响因子：
6.4
作者：
LINDBERG, S;WINGSTRAND, G
通讯作者：
WINGSTRAND, G
从能源和药用植物麻风树的物理坚果根和叶中分离出的富含质膜的囊泡的 H -ATP 酶活性变化，作为盐度的适应机制
DOI：
--
发表时间：
2012
期刊：
影响因子：
--
作者：
Chen JianMiao;Liu Zhaopu;Shao Hongbo;Q. Song;Zheng Qingsong;L. Lian;Mao Yiqing
通讯作者：
Mao Yiqing