Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue
Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue
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DOI：
10.1016/j.vetimm.2009.06.014
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发表时间：
2009-12-15
影响因子：
1.8
通讯作者：
Deffune, Elenice
中科院分区：
文献类型：
--
作者：
Carvalho, Armando de Mattos;Garcia Alves, Ana Liz;Deffune, Elenice
这项工作的目的是分离和培养源自马脂肪组织的间充质干细胞 (MSC)，并使用以下标记物进行细胞表征：CD90、CD44 和 CD13。在马尾根部采集脂肪组织，然后立即分离和培养 MSC，并通过流式细胞术进行后表征，以使用小鼠抗大鼠 CD90 单克隆抗体 (mAb)、异硫氰酸荧光素 (FITC) 进行种间反应测试，并使用特定 mAb 小鼠抗马 CD13 和小鼠抗马 CD44 进行测试。用于分离和细胞培养的技术被证明是安全可行的。 CD90 mAb 与源自马脂肪组织的 MSC 发生交叉反应，并且随着培养传代次数的增加，CD44 在细胞中显示出更高的表达。尽管标志物CD13在其他涉及不同物种MSC的研究中表达反应，但在实现的实验中没有表现出表达。获得的结果揭示了分离和培养的 MSC 表面的免疫表型特征，将这些细胞分类为可应用于马细胞治疗的有前途的祖细胞类型。 (C) 2009 Elsevier B.V. 保留所有权利。
The purpose of this work was to isolate and cultivate mesenchymal stem cells (MSC) derived from equine adipose tissue and conduct cellular characterization with the following markers: CD90, CD44 and CD13. Adipose tissue collection was performed at the base of the horses' tails, followed by immediate isolation and cultivation of the MSC and posterior characterization by flow cytometry for the interspecies reaction test using mouse anti-rat CD90 monoclonal antibody (mAb), fluorescein isothiocyanate (FITC), and tests with specific mAb mouse anti-horse CD13 and mouse anti-horse CD44. The technique used for isolation and cell cultivation proved to be safe and viable. The CD90 mAb expressed cross-reaction with MSC derived from equine adipose tissue and CD44 showed greater expression in cells as the number of culture passages increased. Although marker CD13 expresses reaction in other studies involving MSC in different species, it presented no expression in the experiment realized. The results obtained revealed the immunophenotypic characterization of the surface of isolated and cultivated MSC, classifying these cells as a promising type of progenitor cells that can be applied in equine cellular therapy. (C) 2009 Elsevier B.V. All rights reserved.