Inhibition of mTOR by rapamycin in the amygdala or hippocampus impairs formation and reconsolidation of inhibitory avoidance memory
Inhibition of mTOR by rapamycin in the amygdala or hippocampus impairs formation and reconsolidation of inhibitory avoidance memory
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DOI：
10.1016/j.nlm.2011.10.002
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发表时间：
2012-01-01
影响因子：
2.7
通讯作者：
Roesler, Rafael
中科院分区：
文献类型：
--
作者：
Jobim, Paulo F. C.;Pedroso, Thiago R.;Roesler, Rafael
哺乳动物靶标雷帕霉素(MTOR)是神经元蛋白质合成的中枢调节因子，与突触可塑性和记忆有关。在这里，我们表明，雷帕霉素抑制基底外侧杏仁核(BLA)或背侧海马区(DH)的mTOR损害了大鼠抑制回避(IA)记忆的形成和重新巩固。在IA训练或恢复之前或之后，雄性Wistar大鼠接受双侧BLA或DH区注射赋形剂或雷帕霉素。在用药后不同时间点检测记忆保持情况。雷帕霉素在训练前或训练后立即给药或恢复到BLA后，会损害长期IA保留。当将雷帕霉素注入脑海马后，在训练前或恢复后立即给予雷帕霉素会造成记忆障碍。提取后雷帕霉素的损害作用需要记忆提取，但不能通过提醒电击来逆转。这些结果首次证明BLA和DH中的mTOR可能在IA记忆再巩固中起作用。(C)2011 Elsevier Inc.保留所有权利。
Mammalian target of rapamycin (mTOR), a central regulator of protein synthesis in neurons, has been implicated in synaptic plasticity and memory. Here we show that mTOR inhibition by rapamycin in the basolateral amygdala (BLA) or dorsal hippocampus (DH) impairs both formation and reconsolidation of memory for inhibitory avoidance (IA) in rats. Male Wistar rats received bilateral infusions of vehicle or rapamycin into the BLA or DH before Or after IA training or retrieval. Memory retention was tested at different time points after drug infusion. Rapamycin impaired long-term IA retention when given before or immediately after training or retrieval into the BLA. When infused into the DH, rapamycin produced memory impairment when given before training or immediately after retrieval. The impairing effects of post-retrieval rapamycin required memory retrieval and were not reversed by a reminder shock. The results provide the first evidence that mTOR in the BLA and DH might play a role in IA memory reconsolidation. (C) 2011 Elsevier Inc. All rights reserved.