Expression and electrophysiological characteristics of P2X3 receptors in interstitial cells of Cajal in rats with partial bladder outlet obstruction
Expression and electrophysiological characteristics of P2X3 receptors in interstitial cells of Cajal in rats with partial bladder outlet obstruction
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DOI：
10.1111/j.1464-410x.2012.11408.x
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发表时间：
2013-05-01
影响因子：
4.5
通讯作者：
Wang, He
中科院分区：
文献类型：
--
作者：
Li, Yuxin;Xue, Li;Wang, He
目的探讨部分膀胱出口梗阻（PBOO）后不同时间点Cajal间质细胞（ICC）嘌呤能P2 X（3）受体的表达及电生理特性。术后4周，进行膀胱测压以研究体内膀胱功能。随后，大鼠在4时被人道处死，分别于造模后6、8周取膀胱标本，免疫荧光染色检测膀胱ICC中P2 X（3）的表达，逆转录-聚合酶链反应（RT-PCR）检测P2 X（3）mRNA的表达。PBOO后相应ICCs的内向电流进行了电生理学研究。PBOO大鼠逼尿肌压力。PBOO组大鼠膀胱重量显著高于对照组，与假手术组相比，PBOO组大鼠ICC中P2 X（3）染色强度显著增加。C-kit标记的ICCs经P2 X（3）抗体染色，RT-PCR显示P2 X（3）mRNA在PBOO大鼠ICCs中的表达在4、6、8周时均显著上调，P2 X（3）mRNA的表达在PBOO大鼠ICCs中呈强阳性，P2 X（3）mRNA的表达在PBOO大鼠ICCs中呈弱阳性。与对照组相比，PBOO组的内向电流的平均峰值幅度显著增加。PBOO可增强P2 X（3）受体的功能，表现为α，β-亚甲基ATP增强的大鼠膀胱ICCs内向电流。
ObjectiveTo investigate the expression and electrophysiological characteristics of purinergic P2X(3) receptors in interstitial cells of Cajal (ICCs) at different time points after partial bladder outlet obstruction (PBOO) in rats.Materials and MethodsIn all, 48 female Sprague-Dawley rats were randomly divided into four groups: 4, 6 and 8 week PBOO groups and sham-operated controls. At 4 weeks after surgery, cystometry was performed to investigate bladder function in vivo. Subsequently, the rats were humanely killed at 4, 6 or 8 weeks and the bladders were harvested for measurements.P2X(3) expression in ICCs of bladder was investigated by immunofluorescent staining.The level of P2X(3) mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR).Inward currents in corresponding ICCs after PBOO were investigated electrophysiologically.ResultsCystometrography showed a valid increase in maximum detrusor pressure in rats subjected to PBOO. The bladder weight in the PBOO group was significantly higher than that in the control group.In contrast to sham rats, there was a significant increase in the intensity of P2X(3) staining in the ICCs in all PBOO groups. C-kit labelled isolated ICCs were strongly stained with the P2X(3) antibody.RT-PCR showed that the expression of P2X(3) mRNA was significantly up-regulated at 4, 6 and 8 weeks in the ICCs from the PBOO rats.In the ICCs, the mean peak amplitude of inward currents was significantly increased in the PBOO groups compared with the control group.ConclusionsThe expression of P2X(3) receptors showed a time dependent up-regulation in the ICCs of the bladder in rats with PBOO.PBOO induced the potentiation of P2X(3) receptors function, as evidenced by alpha, beta-methylene ATP-enhanced inward currents in the ICCs of the rat bladder.