Fluoroimmunoassay of influenza virus using sulfur-doped graphitic carbon nitride quantum dots coupled with Ag2S nanocrystals
Fluoroimmunoassay of influenza virus using sulfur-doped graphitic carbon nitride quantum dots coupled with Ag2S nanocrystals
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DOI：
10.1007/s00604-020-04433-1
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发表时间：
2020-07-21
影响因子：
5.7
通讯作者：
Park, Enoch Y.
中科院分区：
文献类型：
--
作者：
Achadu, Ojodomo J.;Lioe, De Xing;Park, Enoch Y.
采用单源前驱体溶剂热法合成了新型硫掺杂石墨碳氮化物量子点（S-gCNQD）。当激发波长为400 nm时，粒径约为5 nm的S-gCNQD在512 nm处发出强烈的绿色荧光，在水溶液中的量子产率约为33%.将制备的S-gCNQDs和Ag 2S纳米晶作为新型功能材料，分别与特异性抗人甲型流感单克隆抗体偶联，制备了用于病毒检测的生物传感器。在甲型流感病毒的存在下，S-gCNQDs/Ag 2S标记的抗体之间的相互作用导致纳米三明治结构的形成，这伴随着S-gCNQDs的荧光增强。在10 fg/mL至1.0 ng/mL范围内，荧光强度的变化与甲型流感病毒（H1N1）的浓度呈线性关系，检测限为5.5 fg/mL。将该测定法应用于与人血清混合的临床分离的甲型流感病毒（H3 N2/Yokohama）的测定。在H3 N2病毒10（2）- 5 x 10（4）PFU/mL的检测范围内，获得的检测限为100 PFU/mL。
Novel sulfur-doped graphitic carbon nitride quantum dots (S-gCNQDs) are synthesized using a single-source precursor in a one-step solvothermal process. The S-gCNQDs with a size of similar to 5-nm displayed a strong green intrinsic fluorescence at 512 nm when excited at 400 nm, with a quantum yield of similar to 33% in aqueous solution. The prepared S-gCNQDs and Ag2S nanocrystals were applied as innovative functional materials to fabricate a biosensor for virus detection based on the conjugation of specific anti-human influenza A monoclonal antibody to the S-gCNQDs and Ag2S NCs, respectively. In the presence of the influenza A virus, an interaction between the S-gCNQDs/Ag2S-labeled antibody resulted in the formation of a nanosandwich structure, which is accompanied by the fluorescence enhancement of the S-gCNQDs. The change in fluorescence intensity linearly correlats with the concentration of the influenza A virus (H1N1) in the 10 fg/mL to 1.0 ng/mL range, with a limit of detection of 5.5 fg/mL. The assay was applied to the assay of clinically isolated influenza A virus (H3N2/Yokohama) mixed with human serum. The obtained limit of detection was 100 PFU/mL within the detection range of 10(2)- 5 x 10(4)PFU/mL for the H3N2 virus.