Transcriptomic analysis reveals the role of FOUR LIPS in response to salt stress in rice
Transcriptomic analysis reveals the role of FOUR LIPS in response to salt stress in rice
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DOI：
10.1007/s11103-022-01282-9
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发表时间：
2022-05
影响因子：
5.1
通讯作者：
Chunxia Zhang;Jie Zhang;Huichao Liu;Xiaoxiao Qu;Junxue Wang;Qixiumei He;Junjie Zou;Kezhen Yang-Kezhen-Ya
中科院分区：
文献类型：
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作者：
Chunxia Zhang;Jie Zhang;Huichao Liu;Xiaoxiao Qu;Junxue Wang;Qixiumei He;Junjie Zou;Kezhen Yang-Kezhen-Ya
关键信息R2 R3-MYB转录因子FOUR LIPS与B型细胞周期蛋白依赖激酶1;1相关，赋予水稻耐盐性。AtFLP在水稻中只有一个直向同源蛋白OsFLP。然而，OsFLP的功能在很大程度上是未知的。在本研究中，我们通过RNA-seq和ChIP-seq来研究OsFLP在水稻中的潜在作用。这些结果表明OsFLP可能是一个多功能的调节因子，参与水稻生长发育和逆境胁迫反应中的许多生物学过程。然而，我们主要集中在OsFLP在盐胁迫响应中的作用。盐胁迫下的表型分析表明，Osflp对盐胁迫表现出明显的敏感性，而OsFlp表达系表现出明显的耐盐性。酵母单杂交和电泳迁移率变动分析（EMSA）结果表明，OsFLP与水稻B型细胞周期蛋白依赖性激酶1;1（OsCDKB 1;1）的启动子区直接结合，并抑制OsCDKB 1; 1在OsFLP中的表达。干扰OsCDKB 1; 1的表达可显著增强水稻对盐胁迫的耐受性。综上所述，本研究揭示了OsFLP对OsCDKB 1; 1的调控在水稻耐盐性中的重要作用，并在很大程度上扩展了对OsFLP在水稻耐盐性中作用的认识。
Key messageAn R2R3-MYB transcription factor FOUR LIPS associated with B-type Cyclin-Dependent Kinase 1;1 confers salt tolerance in rice.AbstractThe Arabidopsis FOUR LIPS (AtFLP), an R2R3 MYB transcription factor, acts as an important stomatal development regulator. Only one orthologue protein of AtFLP,Oryza sativaFLP (OsFLP), was identified in rice. However, the function of OsFLP is largely unknown. In this study, we conducted RNA-seq and ChIP-seq to investigate the potential role of OsFLP in rice. Our results reveal that OsFLP is probably a multiple functional regulator involved in many biological processes in growth development and stress responses in rice. However, we mainly focus on the role of OsFLP in salt stress response. Consistently, phenotypic analysis under salt stress conditions showed thatosflpexhibited significant sensitivity to salt stress, whileOsFLPover-expression lines displayed obvious salt tolerance. Additionally, Yeast one-hybrid assay and electrophoretic mobility shift assay (EMSA) showed that OsFLP directly bound to the promoter region ofOryza sativaB-type Cyclin-Dependent Kinase 1;1 (OsCDKB1;1), and the expression ofOsCDKB1;1was repressed inosflp. Disturbing the expression ofOsCDKB1;1remarkably enhanced the tolerance to salt stress. Taken together, our findings reveal a crucial function of OsFLP regulatingOsCDKB1;1in salt tolerance and largely extend the knowledge about the role of OsFLP in rice.