Sema4d is required for the development of the hindbrain boundary and skeletal muscle in zebrafish.
Sema4d is required for the development of the hindbrain boundary and skeletal muscle in zebrafish.
复制标题
DOI：
10.1016/j.bbrc.2013.02.085
复制
发表时间：
2013-04
影响因子：
3.1
通讯作者：
Jie Yang;Zhen Zeng;Jun-cheng Wei;Lijun Jiang;Quanfu Ma;Mingfu Wu;Xiao-yuan Huang;Shuangmei Ye;Ye Li;D. Ma;Q. Gao
中科院分区：
文献类型：
--
作者：
Jie Yang;Zhen Zeng;Jun-cheng Wei;Lijun Jiang;Quanfu Ma;Mingfu Wu;Xiao-yuan Huang;Shuangmei Ye;Ye Li;D. Ma;Q. Gao
Semaphorin 4d（SEMA 4D），也称为CD 100，是一种少突胶质细胞分泌的R-Ras GTP酶激活蛋白（GAP），影响轴突生长，涉及一系列过程，包括细胞粘附、运动、血管生成、免疫应答和肿瘤进展。然而，其实际的生理机制及其在发育中的作用仍不清楚。本研究的重点是sema 4d在斑马鱼胚胎发育和表达模式中的作用，以及使用sema 4d特异性反义吗啉代寡核苷酸抑制其对发育的影响。在这项研究中，在所有发育阶段表达的sema 4d的敲除导致斑马鱼胚胎的后脑和躯干结构缺陷。此外，这些表型似乎与三个后脑菱体区边界标记物wnt 1、epha 4a和foxb1.2以及两个肌源性调节因子myod和myog的异常表达有关。此外，细胞凋亡的显着增加出现在sema 4d敲低胚胎，而细胞增殖没有观察到明显的减少。总的来说，这些数据表明，sema 4d在后脑和骨骼肌的发育中起着重要的作用。
Semaphorin4d (SEMA4D), also known as CD100, an oligodendrocyte secreted R-Ras GTPase-activating protein (GAP), affecting axonal growth is involved in a range of processes including cell adhesion, motility, angiogenesis, immune responses and tumour progression. However, its actual physiological mechanisms and its role in development remain unclear. This study has focused on the role of sema4d in the development and expression patterns in zebrafish embryos and the effect of its suppression on development using sema4d-specific antisense morpholino-oligonucleotides. In this study the knockdown of sema4d, expressed at all developmental stages, lead to defects in the hindbrain and trunk structure of zebrafish embryos. In addition, these phenotypes appeared to be associated with the abnormal expression of three hindbrain rhombomere boundary markers, wnt1, epha4a and foxb1.2, and two myogenic regulatory factors, myod and myog. Further, a notable increase of cell apoptosis appeared in the sema4d knockdown embryos, while no obvious reduction in cell proliferation was observed. Collectively, these data suggest that sema4d plays an important role in the development of the hindbrain and skeletal muscle.