A rationale for the application of trasylol as a protease inhibitor in radioimmunoassay.
A rationale for the application of trasylol as a protease inhibitor in radioimmunoassay.
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trasylol 作为蛋白酶抑制剂在放射免疫分析中应用的基本原理。
DOI：
10.1016/0024-3205(81)90291-5
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发表时间：
1981
期刊：
影响因子：
6.1
通讯作者：
Zyznar,ES
中科院分区：
文献类型：
--
作者：
Zyznar,ES
通过放射免疫测定法测量生物体液中的多肽激素水平常常受到配体的蛋白水解降解的损害，这降低了配体的量，改变了其与抗体结合的亲和力或增加了标记配体的非特异性结合。为了避免这些困难，在样品收集、放射免疫测定孵育期和放射性标记储存期间使用蛋白酶抑制剂。特雷西洛θ与EDTA联用具有许多特性，使其成为适用于放射免疫分析的蛋白水解抑制剂，因此在放射免疫分析中得到广泛应用。这些特点进行了讨论。
Measurements of polypeptide hormone levels in biological fluids by radioimmunoassay are often impaired by proteolytic degradation of the ligand which decreases the amount of ligand, alters its affinity of binding to antibody or increases nonspecific binding of labelled ligand. To circumvent these difficulties, protease inhibitors have been employed during sample collection, radioimmunoassay incubation periods and radiolabel storage. Trasylolθin combination with EDTA possesses many characteristics which make it a suitable proteolytic inhibitor for application to radioimmunoassay and, consequently, has attained widespread use in radioimmunoassays. These characteristics are discussed.