Effects of Sphaeropsis Blight on Rhizosphere Soil Bacterial Community Structure and Soil Physicochemical Properties of Pinus sylvestris var. mongolica in Zhanggutai, China
Effects of Sphaeropsis Blight on Rhizosphere Soil Bacterial Community Structure and Soil Physicochemical Properties of Pinus sylvestris var. mongolica in Zhanggutai, China
复制标题
白粉病对樟子松根际土壤细菌群落结构及土壤理化性质的影响
DOI：
10.3390/f10110954
复制
发表时间：
2019-11-01
期刊：
影响因子：
2.9
通讯作者：
Song, Ruiqing
中科院分区：
文献类型：
--
作者：
Halifu, Saiyaremu;Deng, Xun;Song, Ruiqing
樟子松蒙古树种生长速度快，抗逆性强，是生态建设和环境修复的重要树种。樟子松沙棘枯萎病是由沙棘枯萎病菌引起的一种广泛流行的病害。本研究以未感病(CK)和感病(SS)樟子松为研究对象。中国在辽宁省章古台地区种植蒙古植物。Illumina高通量测序是一种广泛使用的方法，它以合成测序为模板，结合可逆终止子。在本研究中，评估了根际土壤理化性质、细菌群落结构、多样性细菌属和α多样性指数之间关系的系统差异。研究结果表明：(1)SS土壤香农指数显著高于对照，5月份显著低于7月份和9月份(p<；0.05)。(2)非度量多维尺度(NMDS)显示细菌群落结构在5月(春季)、7月(夏季)和9月之间存在差异。(3)在门水平上，三个季节CK和SS土壤中的细菌属没有显著差异，而在属水平上，大约有19个不同细菌属。19个不同细菌属与环境因子和α多样性的相关性研究表明，未侵染和侵染樟子松的细菌属之间存在差异。蒙古的分布也不同。土壤细菌属与土壤理化性质呈正相关，与SS呈负相关。综上所述，樟子松根际土壤养分和微生物群落结构的差异。蒙古枯梢病是引起枯梢病的主要病原。
Pinus sylvestris var. mongolica is an important tree species for ecological construction and environmental restoration owing to its rapid growth rate and excellent stress resistance. Pinus sylvestris var. mongolica sphaeropsis blight is a widespread disease caused by Sphaeropsis sapinea. This study was focused on non-infected (CK) and infected (SS) Pinus sylvestris var. mongolica plants in Zhanggutai area, Liaoning Province, China. Illumina high-throughput sequencing based on the templates of sequencing-by-synthesis working with reversible terminators is a widely used approach. In the present study, systematic differences in relationships among rhizosphere soil physicochemical properties, bacterial community structure, diverse bacterial genera, and alpha diversity indices between the two categories were evaluated. The current findings are as follows: (1) Shannon's index of SS soil was significantly higher than CK, and it was significantly lower in May than July and September (p < 0.05). (2) Non-metric multidimensional scaling (NMDS) showed a difference in bacterial community structure during May (spring), July (summer), and September. (3) At the phylum level, no significant difference was found in the bacterial genera between CK and SS soil for three seasons; however, at the genus level, there were about 19 different bacterial genera. The correlation studies between 19 different bacterial genera and environmental factors and alpha-diversity indicated that bacterial genera of non-infected and infected Pinus sylvestris var. mongolica were distributed differently. The bacterial genera with CK were positively correlated with soil physicochemical properties, while a negative correlation was found for SS. In conclusion, the differences in nutrient and microbial community structure in the rhizosphere soil of Pinus sylvestris var. mongolica are the main causes of shoot blight disease.