AUG codons at the beginning of protein coding sequences are frequent in eukaryotic mRNAs with a suboptimal start codon context
AUG codons at the beginning of protein coding sequences are frequent in eukaryotic mRNAs with a suboptimal start codon context
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DOI：
10.1093/bioinformatics/bti136
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发表时间：
2005-04-01
期刊：
影响因子：
5.8
通讯作者：
Kochetov, AV
中科院分区：
文献类型：
--
作者：
Kochetov, AV
目的：翻译起始位点在真核生物mRNA的翻译效率控制中起着重要作用。然而，具有起始AUG密码子的次优背景的mRNA相对丰富。这是可能的，至少有一些mRNA与次优的起始密码子上下文包含其他信号提供额外的信息，为有效的AUG recognition.Results：AUG密码子的频率在真核生物mRNA的编码部分的开始进行了分析，在翻译起始密码子的上下文。发现在具有最佳起始密码子背景的mRNA中观察到的下游AUG含量接近预期值，而在具有次优背景的mRNA中观察到的下游AUG含量显著更高。下游AUG密码子可能通常可以用作额外的起始位点，以增加具有注释的起始密码子的次优背景的mRNA的翻译速率，并且许多真核蛋白质可以通过一些N-末端异质性来表征。
Motivation: The translation start site plays an important role in the control of translation efficiency of eukaryotic mRNAs. However, mRNAs with a suboptimal context of start AUG codon are relatively abundant. It is likely that at least some mRNAs with suboptimal start codon context contain the other signals providing additional information for efficient AUG recognition.Results: Frequency of AUG codons at the beginning of the coding part of eukaryotic mRNAs was analyzed in relation to the context of translation start codon. It was found that the observed downstream AUG content in the mRNAs with optimal start codon context was close to the expected value, whereas it was significantly higher in the mRNAs with a suboptimal context. It is likely that downstream AUG codons can often be utilized as additional start sites to increase translation rate of mRNAs with a suboptimal context of the annotated start codon and many eukaryotic proteins can be characterized by some N-end heterogeneity.