Regional mapping of the creatine kinase b (CKBB) gene in rabbit (Oryctolagus cuniculus) and man using a rat cDNA probe.
Regional mapping of the creatine kinase b (CKBB) gene in rabbit (Oryctolagus cuniculus) and man using a rat cDNA probe.
复制标题
使用大鼠 cDNA 探针对兔 (Oryctolagus cuniculus) 和人的肌酸激酶 b (CKBB) 基因进行区域定位。
DOI：
10.1159/000132615
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Martin-DeLeon,PA
中科院分区：
文献类型：
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作者：
Mahoney,CE;Picciano,SR;Burton,KM;Martin-DeLeon,PA
使用大鼠肌酸激酶（脑形式）cDNA 克隆进行原位杂交，我们将该基因定位于人和兔补体中。数据分析显示，人类的基因座位于 14q32，证实了之前基于体细胞杂交研究和 Southern blot 分析的分配。在兔子中，20q13→qter 上的显着积累以及染色体末端的主要标记为该基因在该位点的定位提供了证据。大鼠探针与人和兔中期细胞的异源杂交强调了该酶序列的高度保守性。
Using a rat creatine kinase (brain form) cDNA clone for in situ hybridization, we have localized the gene in both the human and the rabbit complement. An analysis of the data shows that the locus in the human is at 14q32, confirming previous assignments based on somatic hybridization studies and Southern blot analysis. In the rabbit, significant accumulation on 20q13→qter with the predominant labeling at the end of the chromosome provides evidence for the localization of the gene at this site. The heterologous hybridizations of a rat probe to both human and rabbit metaphases underscore the highly conserved nature of the sequences for this enzyme.