An extended wave of global mRNA deadenylation sets up a switch in translation regulation across the mammalian oocyte-to-embryo transition.
An extended wave of global mRNA deadenylation sets up a switch in translation regulation across the mammalian oocyte-to-embryo transition.
复制标题
整体 mRNA 去腺苷化的延长波在哺乳动物卵母细胞到胚胎的转变过程中建立了翻译调控的开关。
DOI：
10.1016/j.celrep.2024.113710
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发表时间：
2024
期刊：
影响因子：
8.8
通讯作者：
Cook-Andersen,Heidi
中科院分区：
文献类型：
--
作者：
Lee,Katherine;Cho,Kyucheol;Morey,Robert;Cook-Andersen,Heidi
在没有新的转录的情况下，卵母细胞到胚胎转变（OET）的基因表达依赖于mRNA poly（A）尾的调节来控制翻译。然而，在哺乳动物中，尾部动力学如何在OET中塑造翻译仍然不清楚。我们进行长读段RNA测序，以揭示小鼠OET中的poly（A）尾长度，并结合已发表的核糖体分析数据，提供对poly（A）尾和整个过渡翻译的综合性全转录组分析。我们发现了在受精过程中，短尾的卵母细胞沉积的mRNA在没有多聚腺苷酸化的情况下通过对去腺苷酸化的抗性被激活。随后，在胚胎中，mRNA被重新腺苷化，并在全球多聚腺苷酸化的激增中翻译。我们进一步确定了同工型水平上poly（A）尾长的调节以及受调节转录物中mRNA序列基序的阶段特异性富集。这些数据提供了深入了解多聚腺苷酸尾调控的阶段特异性机制，该机制协调了哺乳动物从卵母细胞到胚胎的基因表达。
Without new transcription, gene expression across the oocyte-to-embryo transition (OET) relies instead on regulation of mRNA poly(A) tails to control translation. However, how tail dynamics shape translation across the OET in mammals remains unclear. We perform long-read RNA sequencing to uncover poly(A) tail lengths across the mouse OET and, incorporating published ribosome profiling data, provide an integrated, transcriptome-wide analysis of poly(A) tails and translation across the entire transition. We uncover an extended wave of global deadenylation during fertilization in which short-tailed, oocyte-deposited mRNAs are translationally activated without polyadenylation through resistance to deadenylation. Subsequently, in the embryo, mRNAs are readenylated and translated in a surge of global polyadenylation. We further identify regulation of poly(A) tail length at the isoform level and stage-specific enrichment of mRNA sequence motifs among regulated transcripts. These data provide insight into the stage-specific mechanisms of poly(A) tail regulation that orchestrate gene expression from oocyte to embryo in mammals.