Interkingdom adenosine signal reduces Pseudomonas aeruginosa pathogenicity.
Interkingdom adenosine signal reduces Pseudomonas aeruginosa pathogenicity.
复制标题
界间腺苷信号降低铜绿假单胞菌的致病性。
DOI：
10.1111/j.1751-7915.2012.00338.x
复制
发表时间：
2012
影响因子：
5.7
通讯作者：
Wood,ThomasK
中科院分区：
文献类型：
--
作者：
Sheng,Lili;Pu,Mingming;Hegde,Manjunath;Zhang,Yuanxing;Jayaraman,Arul;Wood,ThomasK
铜绿假单胞菌是一种重要的胃肠道致病菌。在这里，我们证明了腺苷，来自真核宿主的ATP的水解，是这种病原体在胃肠道中的一个强有力的跨王国信号。腺苷的加入几乎消除了P. 铜绿假单胞菌被膜的形成，并通过阻止鼠李糖脂的产生而消除了蜂群现象。由于腺苷代谢产物肌苷不影响生物膜的形成，而且由于不能代谢腺苷的突变体的行为与野生型菌株相似，因此不需要腺苷代谢来降低致病性。腺苷还减少了毒力因子绿青素、弹性酶、胞外多糖、铁载体和假单胞菌喹诺酮信号的产生，从而降低了对秀丽线虫的毒力。为了深入了解腺苷如何降低铜绿假单胞菌的毒力，进行了全转录组分析，发现腺苷的添加抑制了类似于铁充足条件下的基因；然而，腺苷并不直接结合毛皮。因此，腺苷通过抑制与铁获取相关的基因来降低作为跨王国信号的P. 铜绿假单胞菌的致病性。
Pseudomonas aeruginosais becoming recognized as an important pathogen in the gastrointestinal (GI) tract. Here we demonstrate that adenosine, derived from hydrolysis of ATP from the eucaryotic host, is a potent interkingdom signal in the GI tract for this pathogen. The addition of adenosine nearly abolishedP. aeruginosabiofilm formation and abolished swarming by preventing production of rhamnolipids. Since the adenosine metabolite inosine did not affect biofilm formation and since a mutant unable to metabolize adenosine behaved like the wild‐type strain, adenosine metabolism is not required to reduce pathogenicity. Adenosine also reduces production of the virulence factors pyocyanin, elastase, extracellular polysaccharide, siderophores and thePseudomonasquinolone signal which led to reduced virulence withCaenorhabditis elegans. To provide insights into how adenosine reduces the virulence ofP. aeruginosa, a whole‐transcriptome analysis was conducted which revealed that adenosine addition represses genes similar to an iron‐replete condition; however, adenosine did not directly bind Fur. Therefore, adenosine decreasesP. aeruginosapathogenicity as an interkingdom signal by causing genes related to iron acquisition to be repressed.