Early expression of yeast genes affected by chemical stress
Early expression of yeast genes affected by chemical stress
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DOI：
10.1128/mcb.25.5.1860-1868.2005
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发表时间：
2005-03-01
影响因子：
5.3
通讯作者：
Jacq, C
中科院分区：
文献类型：
--
作者：
Lucau-Danila, A;Lelandais, G;Jacq, C
环境胁迫的多样性可能是对转录激活因子和转录机制的主要挑战。为了精确描述酵母为适应化学应激而开发的非常早期的基因组反应，我们对酵母基因表达程序的修改进行了时程分析，该程序紧接在添加抗有丝分裂药物苯菌灵之后。对不同的等基因酵母菌株进行了类似的分析，其中删除了编码相关转录因子的基因，并与有效的生物信息学工具相结合。Yap1和Pdr1，两个著名的应激耐受性的关键介质，似乎是负责非常迅速地建立一个瞬时转录反应，包括119个基因。Yap1在这种反应中起主要作用，在体内结合不自动上调的基因的启动子。我们提出，Yap1核定位和DNA结合是必要的，但不足以引起化学应激反应的特异性。
The variety of environmental stresses is probably the major challenge imposed on transcription activators and the transcriptional machinery. To precisely describe the very early genomic response developed by yeast to accommodate a chemical stress, we performed time course analyses of the modifications of the yeast gene expression program which immediately follows the addition of the antimitotic drug benomyl. Similar analyses were conducted with different isogenic yeast strains in which genes coding for relevant transcription factors were deleted and coupled with efficient bioinformatics tools. Yap1 and Pdr1, two well-known key mediators of stress tolerance, appeared to be responsible for the very rapid establishment of a transient transcriptional response encompassing 119 genes. Yap1, which plays a predominant role in this response, binds, in vivo, promoters of genes which are not automatically up-regulated. We proposed that Yap1 nuclear localization and DNA binding are necessary but not sufficient to elicit the specificity of the chemical stress response.