Regenerative versus reparative healing in tendon: A study of biomechanical and histological properties in fetal sheep
Regenerative versus reparative healing in tendon: A study of biomechanical and histological properties in fetal sheep
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DOI：
10.1114/1.1616931
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发表时间：
2003-11-01
影响因子：
3.8
通讯作者：
Soslowsky, LJ
中科院分区：
文献类型：
--
作者：
Beredjiklian, PK;Favata, M;Soslowsky, LJ
以前的研究表明胎儿组织以再生的方式愈合，没有疤痕形成。本研究的目的是比较成人和胎儿肌腱的愈合特性。使用妊娠80-85天的定时交配妊娠母羊。在胎儿外侧伸肌肌腱中进行部分中间腱切断术，并在母体肢体中进行类似的腱切断术。伤后一周，处死胎儿和成年动物，对肌腱进行组织学和力学评价。进行转化生长因子β亚型1（TGF-β 1）的免疫组织化学染色。组织学上，在成人肌腱的创伤部位可见肉芽组织和炎性细胞的间隙。在胎儿肌腱中，在伤口中未观察到异常，胶原结构重建。TGF-β 1在胎儿伤口中表达较低，但在成人伤口中表达上调。两组的生物力学性能无显著差异。我们确定了损伤的胎儿肌腱的再生愈合特性，而成人肌腱组织愈合与瘢痕形成。胎儿肌腱表现出有限的恢复损伤后的机械性能，并不比成人肌腱在7天。更好地了解胎儿愈合的机制可能会导致新的治疗策略在临床上。(C)2003生物医学工程学会。
Previous studies have shown fetal tissues heal in a regenerative fashion without scar formation. The objective of this study is to compare the healing properties of adult and fetal tendons. Time-mated pregnant ewes at 80-85 days of gestation were utilized. A partial, midsubstance tenotomy was performed in the lateral extensor fetal tendons, and analogous tenotomies were created in the maternal limbs. One week after injury, the fetal and adult animals were sacrificed, and tendons were histologically and mechanically evaluated. Immunohistochemical staining for transforming growth factor beta isoform 1 (TGF-beta1) was performed. Histologically, a gap with granulation tissue and inflammatory cells was visible in the site of wounding in the adult tendons. In the fetal tendons, no abnormalities were noted in the wound, with reconstitution of collagen architecture. TGF-beta1 expression was low in fetal but upregulated in the adult wounds. No significant differences were found in the biomechanical properties between groups. We identified regenerative healing properties in injured fetal tendon, while adult tendon tissue healed reparatively with scar formation. Fetal tendons demonstrated a limited recovery of mechanical properties after injury that was no better than that of the adult tendons at seven days. A better understanding of the mechanisms of fetal healing may lead to novel therapeutic strategies in the clinical setting. (C) 2003 Biomedical Engineering Society.