Protease-digested egg-white products induce oral tolerance in mice but elicit little IgE production upon epicutaneous exposure
Protease-digested egg-white products induce oral tolerance in mice but elicit little IgE production upon epicutaneous exposure
复制标题
蛋白酶消化的蛋清产品可诱导小鼠口服耐受，但经皮暴露后几乎不会产生 IgE
DOI：
10.1016/j.alit.2022.03.006
复制
发表时间：
2022
影响因子：
6.8
通讯作者：
Matsumoto Kenji
中科院分区：
文献类型：
--
作者：
Yamada Ayako;Hasegawa Takanori;Fujieda Mikiya;Morita Hideaki;Matsumoto Kenji
背景：早期食物引入诱导耐受，但表皮暴露，特别是通过湿疹病变，促进IgE致敏。为了安全有效地一级预防鸡蛋过敏，我们检查了几种蛋白酶消化的蛋清（EW）产品的三种特性：1）诱导口服耐受性，防止IgE致敏，2）弱的IgE结合，即使在IgE致敏的小鼠中也可以预防过敏反应，以及3）即使与发炎的皮肤接触，表皮IgE致敏作用也很小。方法：在最佳条件下用多种蛋白酶消化加热的EW。首先，三周龄的BALB/c雌性小鼠灌胃给予EW或每种蛋白酶消化的EW产品，然后腹腔内注射卵清蛋白（OVA）或卵类粘蛋白（OVM）与明矾。测定血清OVA和OVM特异性IgE滴度。其次，用OVA/OVM致敏6周龄小鼠，并在腹腔内施用EW或每种蛋白酶消化的EW后测量直肠温度。第三，将EW或每种蛋白酶消化的EW产品施用于胶带剥离的皮肤，每周3天，持续3周。结果：口服胃蛋白酶消化的EW产品（PDEW）和Thermoase PC 10 F消化的EW产品（TDEW）可显著抑制OVA/OVM特异性IgE的产生。两种产品均未引起OVA/OVM致敏小鼠体温下降（过敏反应）。血清OVA-/OVM特异性IgE水平显着降低小鼠epicuphilis暴露于PDEW或TDEW比EW暴露mice.Conclusions：两种蛋白酶消化的EW显示出潜在的最佳EW产品，为早期引进鸡蛋过敏的一级预防。
Background: Early food introduction induces tolerance, but epicutaneous exposure, especially via eczema lesions, promotes IgE sensitization. Aiming for safe and effective primary prevention of egg allergy, we examined several protease-digested egg-white (EW) products for three properties: 1) induction of oral tolerance that prevents IgE sensitization, 2) weak IgE binding that can prevent allergic reactions even in IgE-sensitized mice, and 3) minimal epicutaneous IgE sensitization even when in contact with inflamed skin.Methods: Heated EW was digested with several proteases under optimal conditions. First, three-week-old BALB/c female mice were intragastrically administered EW or each protease-digested EW product, followed by intraperitoneal ovalbumin (OVA) or ovomucoid (OVM) injection with alum. Serum OVA-and OVM-specific IgE titers were measured. Second, six-week-old mice were sensitized with OVA/OVM, and the rectal temperature was measured after intraperitoneal administration of EW or each protease-digested EW. Third, EW or each protease-digested EW product was applied to the tape-stripped skin for 3 days/week for 3 weeks. Serum OVA-and OVM-specific IgE titers were measured.Results: Orally administered pepsin-digested EW product (PDEW) and Thermoase PC10F-digested EW product (TDEW) significantly suppressed OVA-/OVM-specific IgE production. Neither product elicited a body temperature decline (anaphylaxis) in OVA-/OVM-sensitized mice. Serum OVA-/OVM-specific IgE levels were significantly lower in mice epicutaneously exposed to PDEW or TDEW than in EW-exposed mice.Conclusions: Two protease-digested EWs showed potential as optimal EW products for early introduction for primary prevention of egg allergy.