The Genetic Architecture of Larval Aggregation Behavior in Drosophila
The Genetic Architecture of Larval Aggregation Behavior in Drosophila
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DOI：
10.1080/01677063.2021.1887174
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发表时间：
2020-11
期刊：
影响因子：
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通讯作者：
Ross M McKinney;Y. Ben-Shahar
中科院分区：
文献类型：
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作者：
Ross M McKinney;Y. Ben-Shahar
许多昆虫都表现出聚集等基本的社会行为，这些行为在昆虫的取食和交配生态中起着重要作用。然而，调节昆虫聚集的进化、遗传和生理机制对大多数物种来说仍然是未知的。在这里，我们使用自然种群的果蝇，以确定遗传结构，驱动幼虫聚集进食行为。通过使用定量和反向遗传的方法，我们已经确定了一个复杂的神经遗传网络，在调节幼虫的决定，无论是在孤独或作为一个群体的饲料中发挥作用。来自单个基因的RNAi敲除实验的结果表明，几个被识别的基因代表了遗传网络中的关键节点，该网络决定了进食时的聚集水平。此外，我们发现，一个单一的非编码SNP的基因CG14205，一个假定的酰基转移酶，与mRNA表达降低和增加聚集体的形成，这表明它有一个特定的作用，在抑制聚集行为。我们的研究结果确定，第一次，相互作用，以调节自然发生的聚集在D的水平的遗传成分。黑腹幼虫
Many insect species exhibit basal social behaviors such as aggregation, which play important roles in their feeding and mating ecologies. However, the evolutionary, genetic, and physiological mechanisms that regulate insect aggregation remain unknown for most species. Here, we used natural populations of Drosophila melanogaster to identify the genetic architecture that drives larval aggregation feeding behavior. By using quantitative and reverse genetic approaches, we have identified a complex neurogenetic network that plays a role in regulating the decision of larvae to feed in either solitude or as a group. Results from single gene, RNAi-knockdown experiments show that several of the identified genes represent key nodes in the genetic network that determines the level of aggregation while feeding. Furthermore, we show that a single non-coding SNP in the gene CG14205, a putative acyltransferase, is associated with both decreased mRNA expression and increased aggregate formation, which suggests that it has a specific role in inhibiting aggregation behavior. Our results identify, for the first time, the genetic components which interact to regulate naturally occurring levels of aggregation in D. melanogaster larvae.