Differential analysis of human leukocytes by dielectrophoretic field-flow-fractionation
Differential analysis of human leukocytes by dielectrophoretic field-flow-fractionation
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DOI：
10.1016/s0006-3495(00)76812-3
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发表时间：
2000-05-01
影响因子：
3.4
通讯作者：
Gascoyne, PRC
中科院分区：
文献类型：
--
作者：
Yang, J;Huang, Y;Gascoyne, PRC
人类白细胞的差异分析有许多重要的生物学和医学应用。在这项工作中，介电场流分选（deep - fff）是一种利用细胞密度和介电特性差异的细胞分离技术，用于分离主要人类白细胞亚群（T淋巴细胞和b淋巴细胞、单核细胞和粒细胞）的混合物。分离在一个薄型腔室中进行，该腔室底部表面装有微加工的交叉指状电极阵列，并用在线流式细胞仪对分离性能进行表征。为了研究不同白细胞混合物的最佳分离条件，获得了不同DEP场频率下每种白细胞亚型的洗脱断面图。在适当选择的条件下，T-（或B-）淋巴细胞从单核细胞中分离，T-（或B-）淋巴细胞从粒细胞中分离，单核细胞从粒细胞中分离，均取得了较高的分离性能。deep - fff不涉及细胞标记或细胞修饰步骤，为血液学分析提供了新的方法。
The differential analysis of human leukocytes has many important biological and medical applications. In this work, dielectrophoretic field-flow-fractionation (DEP-FFF), a cell-separation technique that exploits the differences in the density and dielectric properties of cells, was used to separate the mixtures of the major human leukocyte subpopulations (T- and B-lymphocytes, monocytes, and granulocytes). The separation was conducted in a thin chamber equipped with an array of microfabricated interdigitated electrodes on the bottom surface, and the separation performance was characterized by on-line flow cytometry. To investigate optimal separation conditions for different leukocyte mixtures, elution fractograms at various DEP field frequencies were obtained for each leukocyte subtype. With appropriately chosen conditions, high separation performance was achieved in separating T- (or B-) lymphocytes from monocytes, T- (or B-) lymphocytes from granulocytes, and monocytes from granulocytes. DEP-FFF does not involve cell-labeling or cell-modification step, and provides a new approach to hematological analysis.