Response of cementoblast-like cells to mechanical tensile or compressive stress at physiological levels in vitro
Response of cementoblast-like cells to mechanical tensile or compressive stress at physiological levels in vitro
复制标题
成牙骨质细胞样细胞对体外生理水平机械拉伸或压缩应力的反应
DOI：
10.1007/s11033-008-9376-3
复制
发表时间：
2009-09-01
影响因子：
2.8
通讯作者：
Bao, Lina
中科院分区：
文献类型：
--
作者：
Huang, Lan;Meng, Yao;Bao, Lina
为了阐明成牙骨质细胞在牙周炎相关牙根吸收中的作用，本研究试图研究小鼠成牙骨质细胞样细胞是否对机械应力有反应，以及机械力如何调节这些细胞中骨唾液酸蛋白（BSP）和骨桥蛋白（OPN）基因的表达。在该力-负荷模型中，施加不同量级和类型的定义的和可再现的机械负荷长达24 h。除细胞增殖发生短暂的可逆性变化外，BSP和OPN基因转录也发生了显著变化，BSP mRNA表达受到胁迫抑制。2，000 mu菌株3和6小时负荷可上调OPN mRNA的表达，而其他负荷则抑制OPN mRNA的表达，研究还表明，4，000 mu菌株可能比2，000 mu菌株对成牙骨质细胞样细胞产生更大的影响。此外，没有明显的证据表明拉伸和压缩之间的差异。这些结果表明，成牙骨质细胞样细胞是敏感的机械应力，并可能发挥作用，在调节正畸相关的根吸收/修复。
To clarify the role of cementoblast in orthodontic-related root resorption, this study was attempted to examine whether murine cementoblast-like cells are responsive to mechanical stress, and how mechanical forces regulate bone sialoprotein (BSP) and osteopontin (OPN) gene expression in these cells in vitro. In this force-loading model, defined and reproducible mechanical loadings of different magnitudes and types were applied up to 24 h. Besides a transitory and reversible change in cell proliferation, remarkable alterations in gene transcription of BSP and OPN were found. BSP mRNA was suppressed by the stresses. Three and six hours-loadings at 2,000 mu strain up-regulated the expression of OPN mRNA, while the other loadings inhibited it. The study also concluded that 4,000 mu strain was likely to exert more influence on cementoblast-like cells than 2,000 mu strain. Furthermore, no obvious evidence indicated the difference between tension and compression. These results suggested that cementoblast-like cells are sensitive to mechanical stress, and may play a role in regulating orthodontic-related root resorption/repair.