Deduced primary structure of the alpha subunit of the GTP-binding stimulatory protein of adenylate cyclase.
Deduced primary structure of the alpha subunit of the GTP-binding stimulatory protein of adenylate cyclase.
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推断腺苷酸环化酶 GTP 结合刺激蛋白 α 亚基的一级结构。
DOI：
10.1073/pnas.83.5.1251
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发表时间：
1986
影响因子：
11.1
通讯作者：
Gilman,AG
中科院分区：
文献类型：
--
作者：
Robishaw,JD;Russell,DW;Harris,BA;Smigel,MD;Gilman,AG
牛肾上腺cDNA克隆编码整个α亚基的GTP结合调节蛋白，刺激腺苷酸环化酶（GS）的分离和测序。该cDNA指导COS细胞中较大的52 kDa形式的多肽的合成，尽管该克隆似乎编码46 kDa蛋白质。推导的氨基酸序列的Gs α与α亚基的另一个G蛋白，transducin的比较，发现惊人的同源性。
A bovine adrenal cDNA clone encoding the entire alpha subunit of the GTP-binding regulatory protein that stimulates adenylate cyclase (Gs) was isolated and sequenced. This cDNA directed the synthesis of the larger, 52-kDa form of the polypeptide in COS cells, even though the clone appeared to encode a 46-kDa protein. Comparison of the deduced amino acid sequence of Gs alpha with the alpha subunit of another G protein, transducin, revealed striking homologies.