Expression of ICAM-1 and VCAM-1 and monocyte adherence in arteries exposed to altered shear stress.
Expression of ICAM-1 and VCAM-1 and monocyte adherence in arteries exposed to altered shear stress.
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DOI：
10.1161/01.atv.15.1.2
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
P. Walpola;A. Gotlieb;M. Cybulsky;B. Langille
中科院分区：
文献类型：
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作者：
P. Walpola;A. Gotlieb;M. Cybulsky;B. Langille
血流产生的局部剪切应力对循环白细胞与血管内皮的粘附产生直接的机械效应，但其对内皮-白细胞粘附分子表达的影响尚未确定。兔颈动脉切应力在5天内增加170%或减少73%。用抗Rb 1/9单克隆抗体进行面免疫荧光染色，结果显示低切应力下血管细胞粘附分子1（VCAM 1）的表达显著增加，但染色呈斑片状分布。因此，71.4 +/- 7.8%的视野为VCAM-1阳性，而对照动脉中的视野为2.4 +/- 0.47%。通常，大区域显示一致但不均匀的染色。偶尔，细胞的小岛被强烈标记。使用单核细胞特异性抗体HAM 56检测到的单核细胞在低切应力下粘附于内皮; 64.5 +/- 8.2%的单核细胞与可检测到的VCAM-1共定位，尽管许多（83.2 +/- 2.8%）VCAM-1阳性区域缺乏单核细胞。VCAM-1的表达也显着增加，但在较小的程度上，当剪切应力增加约一倍。因此，在高剪切下8.7 +/-1.5%的视野为VCAM-1阳性，而在正常剪切应力下为2.5 +/-0.87%。在高剪切应力下未检测到单核细胞。在正常剪切应力下，使用单克隆抗体Rb 2/3检测到的细胞间粘附分子-1（ICAM-1）广泛分布;因此，53.5 +/- 5.5%的视野含有ICAM-1阳性细胞。细胞的连接区域被重染色。（250字处删节）
Local shear stresses generated by blood flow exert direct mechanical effects on adhesion of circulating leukocytes to vascular endothelium, but their effects on expression of endothelial-leukocyte adhesion molecules have not been determined. Shear stress in rabbit carotid arteries was increased by 170% or decreased by 73% in 5 days by surgical manipulations. En face immunofluorescence staining with the monoclonal antibody Rb1/9 revealed that vascular cell adhesion molecule-1 (VCAM-1) expression was greatly increased under low shear stress, but the distribution of staining was patchy. Thus, 71.4 +/- 7.8% of fields were VCAM-1 positive versus 2.4 +/- 0.47% of fields in control arteries. Frequently, large regions showed consistent but heterogeneous staining. Occasionally, small islands of cells were labeled intensely. Monocytes, detected by use of the monocyte-specific antibody HAM 56, adhered to endothelium under low shear stress; 64.5 +/- 8.2% of the monocytes colocalized with detectable VCAM-1, although many (83.2 +/- 2.8%) VCAM-1-positive regions were devoid of monocytes. VCAM-1 expression also increased significantly but to a lesser extent when shear stress was approximately doubled. Thus, 8.7 +/- 1.5% of fields were VCAM-1 positive under high shear versus 2.5 +/- 0.87% under normal shear stress. No monocytes were detected at high shear stress. At normal shear stresses, intercellular adhesion molecule-1 (ICAM-1), detected by use of the monoclonal antibody Rb2/3, was extensively distributed; thus, 53.5 +/- 5.5% of fields contained ICAM-1-positive cells. The junctional regions of the cells were heavily stained.(ABSTRACT TRUNCATED AT 250 WORDS)