Increased pulmonary microvasuclar permeability induced by alpha-naphthylthiourea.
Increased pulmonary microvasuclar permeability induced by alpha-naphthylthiourea.
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α-萘基硫脲诱导肺微血管通透性增加。
DOI：
10.1152/jappl.1982.52.5.1316
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Taylor,AE
中科院分区：
文献类型：
--
作者：
Rutili,G;Kvietys,P;Martin,D;Parker,JC;Taylor,AE
研究了α -萘基硫脲（ANTU）对开胸麻醉犬肺微血管对血浆蛋白通透性的影响。在结前小肺淋巴管插管后，记录淋巴流量，分析血浆和淋巴中的总蛋白。该方案包括四个实验阶段。期1。在基线期，Jv、淋巴总蛋白、肺动脉压（Ppa）、左房压（Pla）均达到制剂稳定和稳态。期2。Pla增加到大约20 cmH2O，并保持在该水平，直到Jv和蛋白质测量达到新的稳定状态。期3。在Pla降至对照水平后，静脉注射ANTU （5 mg/kg体wt）并测量参数3小时。第4期Pla再次升高至第2期ANTU前的水平，并维持2 - 3小时。ANTU后淋巴总蛋白清除率增加8.6倍，肺毛细血管压力也相应增加。通过估计肺毛细血管膜总蛋白的渗透反射系数（sigma d）来评估血管通透性。anu后Sigma d由0.65降至0.40。从4个实验的血浆蛋白组分中，计算95和280 A的毛细管膜在ANTU后与80和200 A的正常肺毛细血管的等效孔半径。此外，ANTU升高Pla后，血管外肺水从3.8 +/- 0.16增加到5.87 +/- 0.25 (g/g无血干wt), 7.55 +/- 0.55 （g/g无血干wt）。实验设计允许使用淋巴蛋白通量定量评估ANTU后血管通透性增加，由于表面积变化或非肺结构的淋巴污染，误差最小。
The effects of alpha-naphthylthiourea (ANTU) on lung microvascular permeability to plasma proteins were studied in anesthetized open-chest dogs. Lymph flow (Jv) was recorded, and total protein in plasma and lymph was analyzed after cannulating a small prenodal lung lymphatic. The protocol involved four experimental periods. Period 1. During this base-line period the preparation stabilized and steady states were reached in Jv, lymph total protein, pulmonary arterial pressure (Ppa), and left atrial pressure (Pla). Period 2. Pla was increased to approximately 20 cmH2O and maintained at that level until Jv and protein measurements attained a new steady state. Period 3. After Pla was lowered to control levels, ANTU (5 mg/kg body wt) was infused intravenously and parameters were measured for 3 h. Period 4 Pla was again raised to the pre-ANTU levels of period 2 and maintained for an additional 2–3 h. The lymphatic total protein clearance increased 8.6-fold for an equivalent increase in pulmonary capillary pressure after ANTU. Vascular permeability was assessed by estimating the osmotic reflection coefficient (sigma d) for total protein at the pulmonary capillary membrane. Sigma d decreased from 0.65 to 0.40 following ANTU. From plasma protein fractions in four experiments, equivalent pore radii for the capillary membrane of 95 and 280 A were calculated after ANTU compared with 80 and 200 A for normal lung capillaries. In addition, extravascular lung water increased from 3.8 +/- 0.16 to 5.87 +/- 0.25 following ANTU and to 7.55 +/- 0.55 (g/g blood-free dry wt) when Pla was elevated with ANTU. The experimental design allowed quantitative assessment of the vascular permeability increase after ANTU by use of lymph protein fluxes that had minimal errors due to changes in surface area or lymph contamination from nonpulmonary structures.