Effects of deep hypothermic circulatory arrest on the blood brain barrier in a cardiopulmonary bypass model--a pilot study.
Effects of deep hypothermic circulatory arrest on the blood brain barrier in a cardiopulmonary bypass model--a pilot study.
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DOI：
10.1016/j.hlc.2014.04.131
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发表时间：
2014-10
期刊：
影响因子：
--
通讯作者：
Mathew JP
中科院分区：
文献类型：
--
作者：
Bartels K;Ma Q;Venkatraman TN;Campos CR;Smith L;Cannon RE;Podgoreanu MV;Lascola CD;Miller DS;Mathew JP
Neurologic injury is common after cardiac surgery and disruption of the blood brain barrier (BBB) has been proposed as a contributing factor. We sought to study BBB characteristics in a rodent model of cardiopulmonary bypass (CPB) and deep hypothermic circulatory arrest (DHCA). Adult rats were subjected to CPB/DHCA or to sham surgery. Analysis included Western blotting of relevant BBB proteins in addition to in vivo brain magnetic resonance imaging (MRI) using a clinically used low-molecular contrast agent. While quantitative analysis of BBB proteins revealed similar expression levels, MRI showed evidence of BBB disruption after CPB/DHCA compared to sham surgery. Combining molecular BBB analysis and MRI technology in a rodent model is a highly translatable approach to study adverse neurologic outcomes following CPB/DHCA.
