REARRANGEMENT AND TRANSCRIPTION OF A T-CELL RECEPTOR BETA-CHAIN GENE IN DIFFERENT T-CELL SUBSETS
REARRANGEMENT AND TRANSCRIPTION OF A T-CELL RECEPTOR BETA-CHAIN GENE IN DIFFERENT T-CELL SUBSETS
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DOI：
10.1073/pnas.82.2.531
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
DAVIS, MM
中科院分区：
文献类型：
--
作者：
HEDRICK, SM;GERMAIN, RN;DAVIS, MM
功能明确的T淋巴细胞组——辅助性T细胞、细胞毒性T细胞和抑制性T细胞——被检查是否可能与最近发现的T细胞受体有关。受体形成中的基因位点。由于基因重排是功能性基因表达所必需的，从每个组克隆的t细胞系进行了调查，以了解独特的基因重排表达。显示基因重排的细胞系被进一步测试表达成熟的1.2- 1.3千碱基mRNA，这些mRNA是由产生性基因重排转录而来的。辅助T细胞和细胞毒性T细胞可能使用共同的。受体链，而抑制细胞很少这样做，如果有的话。
The functionally defined sets of T lymphocytes-helper T cells, cytotoxic T cells and suppressor T cells-were examined for the possible involvement of a recently identified T-cell receptor .beta. gene locus in receptor formation. Since gene rearrangements are required for functional gene expression, cloned T-cell lines from each of the groups were surveyed for the expression of unique gene rearrangements. Cell lines that showed gene rearrangements were further tested for the expression of the mature 1.2- to 1.3-kilobase mRNA transcribed from a productive gene rearrangement. Helper and cytotoxic T cells may use a common .beta. chain of the receptor, whereas suppressor cells do so rarely, if at all.