Structural characterization, modification and hepatoprotective effects of polysaccharide from Mori Fructus
Structural characterization, modification and hepatoprotective effects of polysaccharide from Mori Fructus
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桑葚多糖的结构表征、修饰及其保肝作用
DOI：
10.1016/j.ijbiomac.2020.02.300
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发表时间：
2020-06-15
影响因子：
8.2
通讯作者：
Zhou, Xin
中科院分区：
文献类型：
--
作者：
Deng, Qingfang;Wang, Xing;Zhou, Xin
本文成功地从桑中分离得到三种纯化的桑多糖（MFP-1、MFP-2和MR-3）。通过氧化降解、硫酸酸化和羧甲基化等结构修饰方法，得到了9种mfp衍生物。结果表明，MFP-1、MFP-2和MFP-3主要由Ara、Gal、Rha、Glc和GalA组成，分子量分别约为20.93 kDa、5623 kDa和118.76 kDa。这些mfp的FT-IR光谱均显示出特征的多糖吸收带，而mfp衍生物由于结构修饰也表现出一定的差异。肝保护活性试验表明，与天然MFP相比，MFP衍生物能显著提高乙醇脱氢酶（ADH）活性。其中，C-MFP的保护作用高于其他两种修饰衍生物。(C) 2020年Elsevier B.V.出版
In this paper, three purified Mori Fnictus (M. Fructus) polysaccharides (MFP-1, MFP-2 and MR-3) were successfully obtained from M. Fnictus. Structural modification methods, including oxidative degradated, sulfated and carboxymethylated modification, were conducted and nine MFPs derivatives were obtained. The molecular structures of MFPs were studied and the results showed that MFP-1, MFP-2 and MFP-3, mainly composed of Ara, Gal, Rha, Glc and GalA, with a molecular weight approximately of 20.93 kDa, 5623 kDa and 118.76 kDa, respectively. FT-IR spectrum of these MFPs all revealed the characteristic polysaccharide absorption bands, while the MFPs derivatives also exhibited some differences due to the structure modification. Hepatoprotective activities tests showed that MFP derivatives could significantly improve the alcohol dehydrogenase (ADH) activity by comparing with the native MFP. And among them, the C-MFP exhibited higher protection effect than the other two modification derivatives. (C) 2020 Published by Elsevier B.V.