Massive horizontal gene transfer in bdelloid rotifers
Massive horizontal gene transfer in bdelloid rotifers
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DOI：
10.1126/science.1156407
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发表时间：
2008-05-30
期刊：
影响因子：
56.9
通讯作者：
Arkhipova, Irina R.
中科院分区：
文献类型：
--
作者：
Gladyshev, Eugene A.;Meselson, Matthew;Arkhipova, Irina R.
仅在少数物种中记录了后生动物的水平基因转移，并且通常与内共生或寄生有关。相比之下，在蛭形轮虫中，我们发现许多基因似乎起源于细菌、真菌和植物，集中在端粒区域以及不同的可移动遗传元件。然而，蛭形近端基因丰富的区域似乎缺乏外源基因，因此与模型后生动物的基因相似。一些外源基因有缺陷，而另一些则完整并可转录；后者中的一些含有功能性剪接体内含子。一个这样的基因，显然是细菌来源的，在大肠杆菌中过度表达并产生了一种活性酶。外源基因的捕获和功能同化可能代表了蛭形动物进化的重要力量。
Horizontal gene transfer in metazoans has been documented in only a few species and is usually associated with endosymbiosis or parasitism. By contrast, in bdelloid rotifers we found many genes that appear to have originated in bacteria, fungi, and plants, concentrated in telomeric regions along with diverse mobile genetic elements. Bdelloid proximal gene- rich regions, however, appeared to lack foreign genes, thereby resembling those of model metazoan organisms. Some of the foreign genes were defective, whereas others were intact and transcribed; some of the latter contained functional spliceosomal introns. One such gene, apparently of bacterial origin, was overexpressed in Escherichia coli and yielded an active enzyme. The capture and functional assimilation of exogenous genes may represent an important force in bdelloid evolution.