Expression of fibrillar types I and III and basement membrane collagen type IV genes in myocardium of tight skin mouse.
Expression of fibrillar types I and III and basement membrane collagen type IV genes in myocardium of tight skin mouse.
复制标题
紧皮小鼠心肌中 I 型和 III 型纤维以及 IV 型基底膜胶原蛋白基因的表达。
DOI：
10.1093/cvr/24.7.578
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发表时间：
1990
影响因子：
10.8
通讯作者：
Eghbali,M
中科院分区：
文献类型：
--
作者：
Chapman,D;Eghbali,M
研究目的-目的是研究致密皮肤小鼠心脏中纤维胶原I型和III型以及基底膜IV型胶原的表达，设计-通过使用α2（I）、α1（III）和α2（IV）前胶原和北方和斑点印迹分析。实验材料-使用1.5-2月龄的C57 BL/6品系的杂合雄性（TSK/+）和正常（+/+）小鼠。测量和结果-北方和斑点印迹分析的结果显示I型胶原的mRNA水平增加了41%，心室肌中III型胶原mRNA水平增加63%，IV型胶原增加33（右心室和左心室）与年龄匹配的纯合（+/+）同窝出生的小鼠中的对应物相比。β肌动蛋白mRNA水平无显著增加。免疫荧光显微镜和I型，III型和IV型胶原的单特异性抗体被用来检查胶原沉积。结果表明，与正常心脏相比，在致密皮肤小鼠心脏的血管周围区域中，I型胶原纤维更厚且更致密。III型纤维没有异常积累observed.Conclusions-紧皮肤小鼠的心脏可能是一个合适的模型，用于研究心脏胶原基因表达的上调及其对心肌疾病的潜在贡献。
Study objective– The aim was to study the expression of fibrillar collagen types I and III and basement membrane type IV collagen in the heart of the tight skin mouse, a genetic mutant with collagen overproduction in various organs.Design– Collagen gene expression was measured in the ventricular tissues of the heart of the tight skin mouse and the age matched homozygous (+/+) litter mates by the use of cDNA probes to α2 (I), α1(III) and α2(IV) procollagen and northern and dot blot analysis. Collagen deposition was examined by immunofluorescent light microscopy using monospecific antibodies to types I, III and IV collagens.Experimental material– Heterozygous male (TSK/+) and normal (+/+) mice, 1.5–2 months old of the C57BL/6 strain were used. The animals were anaesthetised and the hearts were rapidly excised and processed for RNA extraction and antibody staining.Measurements and results– The results of northern and dot blot analyses showed a 41% increase in mRNA level for collagen type I, a 63% increase in mRNA level for type III and a 33% increase in type IV collagen in the ventricular myocardium (right and left ventricles) of the tight skin mouse compared to its counterpart in age matched homozygous (+/+) litter mates. mRNA levels for β actin showed no significant increase. Immunofluorescent light microscopy and monospecific antibodies to types I, III and IV collagens were used to examine collagen deposition. The results showed that collagen type I fibres are thicker and denser in perivascular areas of the tight skin mouse heart compared to normal heart. No abnormal accumulation of type III fibres was observed.Conclusions– The heart of the tight skin mouse may be an appropriate model for studying the up regulation of cardiac collagen gene expression and its potential contribution to myocardial diseases.