PENICILLINASE PLASMID-LINKED GENETIC-DETERMINANTS FOR ENTEROTOXIN-B AND ENTEROTOXIN-C1 PRODUCTION IN STAPHYLOCOCCUS-AUREUS
PENICILLINASE PLASMID-LINKED GENETIC-DETERMINANTS FOR ENTEROTOXIN-B AND ENTEROTOXIN-C1 PRODUCTION IN STAPHYLOCOCCUS-AUREUS
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DOI：
10.1128/iai.47.2.514-521.1985
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
SARID, S
中科院分区：
文献类型：
--
作者：
ALTBOUM, Z;HERTMAN, I;SARID, S
基因编码。-内酰胺酶（bla+）和对金属离子（Cd、Hg、Pb、砷酸盐和亚砷酸盐）的抗性均位于临床菌株金黄色葡萄球菌6344的56.2千碱基质粒pZA10中。该菌株产生肠毒素B和肠毒素C1。通过十二烷基硫酸钠或热处理（43℃）消除pZA10。C)导致细菌丧失产生肠毒素B和肠毒素C1的能力。用BamHI、SalI和BglII限制性内切酶构建了pZA10的物理图谱。以金黄色葡萄球菌RN450或治愈的金黄色葡萄球菌6344为受体，用pZA10 DNA转化分离出耐青霉素、产肠毒素B和产c1的共转化子。转移的质粒表现出遗传不稳定性，表现为限制性模式和分子大小的变化，质粒DNA的丢失和染色体DNA的添加。肠毒素B的产生与这种重排质粒携带的18.1千碱基pZA10片段有关。在经过热处理的金黄色葡萄球菌6344中检测到bla+与金属离子抗性遗传决定因素的染色体协整性，以及肠毒素B和C1的产生。利用含有整合质粒的染色体DNA进行转化，在转化子中切除并重建pza10相关质粒。pZA10与Cd相关的抗性在pZA10整合到宿主染色体上时丢失，在携带切除质粒的转化子中重新出现。
The genes encoding for .beta.-lactamase (bla+) and resistance to metallic ions (Cd, Hg, Pb, arsenate and arsenite) were located in a 56.2-kilobase plasmid, pZA10, isolated from a clinical strain, S. aureus 6344. This strain produced enterotoxin B and enterotoxin C1. Elimination of pZA10 by either sodium dodecyl sulfate or heat treatment (43.degree. C) resulted in the loss of the capability of the bacteria to produce both enterotoxin B and enterotoxin C1. A physical map of pZA10 was constructed with BamHI, SalI and BglII restriction endonucleases. Penicillin-resistant, enterotoxin B- and C1-producing cotransformants were isolated by transformation with pZA10 DNA with either S. aureus RN450 or cured S. aureus 6344 as recipients. The transferred plasmids exhibited genetic instability shown by changes in restriction pattern and molecular size, loss of plasmid DNA and addition of chromosomal DNA. Enterotoxin B production was related to a 18.1-kilobase pZA10 fragment carried by such a rearranged plasmid. Chromosomal cointegration of bla+ with genetic determinants for metallic ion resistance and enterotoxin B and C1 production were detected in heat-treated S. aureus 6344. Transformation employing chromosomal DNA containing the integrated plasmid resulted in excision and reestablishment of pZA10-related plasmids in the transformants. pZA10-linked resistance to Cd which was lost upon the integration of pZA10 into the host chromosome, reappeared in transformants carrying the excised plasmid.