INSULIN GENE ENHANCER BINDING-PROTEIN ISL-1 IS A MEMBER OF A NOVEL CLASS OF PROTEINS CONTAINING BOTH A HOMEODOMAIN AND A CYS-HIS DOMAIN
INSULIN GENE ENHANCER BINDING-PROTEIN ISL-1 IS A MEMBER OF A NOVEL CLASS OF PROTEINS CONTAINING BOTH A HOMEODOMAIN AND A CYS-HIS DOMAIN
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DOI：
10.1038/344879a0
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发表时间：
1990-04-26
期刊：
影响因子：
64.8
通讯作者：
EDLUND, T
中科院分区：
文献类型：
--
作者：
KARLSSON, O;THOR, S;EDLUND, T
大鼠胰岛素I基因增强子的活性主要依赖于双向作用蛋白结合结构域1 -3。在这里，我们报告的分离的互补DNA编码的蛋白质，Isl-1，结合到这些域之一。Isl-1含有一个同源结构域，与由mec-3（参考文献4）和lin-11（参考文献5）编码的秀丽隐杆线虫蛋白质的同源结构域最相似。此外，Isl-1与elin-11和mec-3基因产物一样，含有一个新的Cys-His结构域，这使人联想到已知的金属结合区。这些蛋白质共同定义了一类新的蛋白质，其含有同源结构域和Cys His结构域。Isl-1优先在胰腺内分泌来源的细胞中表达。如果Isl-1和秀丽隐杆线虫基因产物之间的结构同源性反映了功能相似性，那么可以设想Isl-1在胰腺内分泌细胞发育中的作用。
THE activity of the rat insulin I gene enhancer is mainly dependent on twocis-acting protein-binding domains1–3. Here we report the isolation of a complementary DNA encoding a protein, Isl-1, that binds to one of these domains. Isl-1 contains a homeodomain with greatest similarity to those of theCaenorhabditis elegansproteins encoded bymec-3(ref. 4) andlin-11(ref. 5). In addition, Isl-1, like thelin-11andmec-3gene products, contains a novel Cys–His domain which is reminiscent of known metal-binding regions. Together these proteins define a novel class of proteins containing both a homeo- and a Cys His–domain. Isl-1 is preferentially expressed in cells of pancreatic endocrine origin. If the structural homologies between Isl-1 and theC.elegansgene products reflect functional similarities, a role for Isl-1 in the development of pancreatic endocrine cells could be envisaged.